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Candida orthopsilosis and Candida metapsilosis spp. nov. To Replace Candida parapsilosis Groups II and III
TLDR
Cluster analysis of sequence polymorphisms from COX3, SADH, and SYA1, which were common to the three groups, consistently separated the isolates into three distinct sets, suggest the subgroups should be afforded species status.
Molecular Phylogenetics of Candida albicans
TLDR
Analysis of data on multilocus sequence typing, ABC typing, mating type-like locus (MAT) status, and antifungal susceptibility for a panel of 1,391 Candida albicans isolates suggested that isolates had mixed evolutionary histories resembling those of a sexually reproducing species.
Multilocus Sequence Typing for Differentiation of Strains of Candida tropicalis
TLDR
Five of six isolates in one cluster that may acquire clade status were resistant to flucytosine, and this study adds C. tropicalis to Candida albicans and Candida glabrata as Candida species for which a multilocus sequence typing (MLST) system has been set up.
Population Structure and Properties of Candida albicans, as Determined by Multilocus Sequence Typing
TLDR
A significantly higher proportion of isolates resistant to fluconazole, itraconazoles, and flucytosine were homozygous at the MTL, suggesting that antifungal pressure may trigger a common mechanism that leads both to resistance and to MTL homozygosity.
Multilocus Sequence Typing of the Pathogenic Fungus Aspergillus fumigatus
TLDR
Results strongly indicate that A. fumigatus is a species of a relatively recent origin, with low levels of sequence dissimilarity, and Typing methods based on variable numbers of tandem repeats offer higher levels of strain discrimination.
Candida albicans Strain Maintenance, Replacement, and Microvariation Demonstrated by Multilocus Sequence Typing
TLDR
MLST is proposed to propose that some C. albicans isolates, or all isolates under yet-to-be-determined conditions, maintain a high level of genetic diversity by mechanisms such as recombination, gene conversion, or chromosomal ploidy change.
Collaborative Consensus for Optimized Multilocus Sequence Typing of Candida albicans
TLDR
Analysis of data for all possible combinations of five, six, seven, eight, and nine of the fragments showed that a set comprising the fragments AAT1a, ACC1, ADP1, MPIb, SYA1, VPS13, and ZWF1b was the smallest that yielded 86 unique diploid sequence types for the 86 isolates.
Genotyping of Candida orthopsilosis Clinical Isolates by Amplification Fragment Length Polymorphism Reveals Genetic Diversity among Independent Isolates and Strain Maintenance within Patients
TLDR
AFLP was proven to unambiguously identify C. orthopsilosis at the species level and efficiently delineate intraspecific genetic relatedness, and a successful strain colonization within the same patient occurred, as revealed by strain maintenance in various body sites.
Optimization and Validation of Multilocus Sequence Typing for Candida albicans
TLDR
Previous MLST results for C. albicans are validated and an optimized system based on sequencing eight gene fragments for routine MLST with this species is proposed, underlining the power of MLST to differentiate isolates for epidemiological studies.
Differential Requirement of the Transcription Factor Mcm1 for Activation of the Candida albicans Multidrug Efflux Pump MDR1 by Its Regulators Mrr1 and Cap1
TLDR
A differential requirement for the coregulator Mcm1 for Cap1- and Mrr1-mediated MDR1 upregulation is demonstrated, which provides more detailed insight into the molecular mechanisms of drug resistance in this important human fungal pathogen.
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