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CCAN Makes Multiple Contacts with Centromeric DNA to Provide Distinct Pathways to the Outer Kinetochore
Kinetochore specification and assembly requires the targeted deposition of specialized nucleosomes containing the histone H3 variant CENP-A at centromeres. However, CENP-A is not sufficient to driveExpand
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Induced Ectopic Kinetochore Assembly Bypasses the Requirement for CENP-A Nucleosomes
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. Although prior work identified the centromeric histone H3-variant CENP-A as the importantExpand
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CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold
The multiprotein kinetochore complex must assemble at a specific site on each chromosome to achieve accurate chromosome segregation. Defining the nature of the DNA-protein interactions that specifyExpand
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The architecture of CCAN proteins creates a structural integrity to resist spindle forces and achieve proper Intrakinetochore stretch.
Constitutive centromere-associated network (CCAN) proteins, particularly CENP-C, CENP-T, and the CENP-H/-I complex, mechanically link CENP-A-containing centromeric chromatin within the innerExpand
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A quantitative description of Ndc80 complex linkage to human kinetochores
The Ndc80 complex, which mediates end-on attachment of spindle microtubules, is linked to centromeric chromatin in human cells by two inner kinetochore proteins, CENP-T and CENP-C. Here to quantifyExpand
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Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins
The N and C termini of CENP-T undergo tension-dependent separation, suggesting that CENP-T elongation is responsible for changes in the shape of the inner kinetochore.
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The CENP-S complex is essential for the stable assembly of outer kinetochore structure
The constitutive centromere-associated network (CCAN) proteins are central to kinetochore assembly. To define the molecular architecture of this critical kinetochore network, we sought to determineExpand
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Spindle assembly checkpoint proteins are positioned close to core microtubule attachment sites at kinetochores
Depletion analyses and nanometer-scale mapping of spindle assembly checkpoint proteins reveal how these proteins are integrated within the substructure of the kinetochore.
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Prolonged Mitosis of Neural Progenitors Alters Cell Fate in the Developing Brain
Embryonic neocortical development depends on balanced production of progenitors and neurons. Genetic mutations disrupting progenitor mitosis frequently impair neurogenesis; however, the link betweenExpand
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How the kinetochore couples microtubule force and centromere stretch to move chromosomes
The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-endExpand
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