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Purification and Characterization of Chitinase B from Moderately Thermophilic Bacterium Ralstonia sp. A-471
Chitinase B was purified from a culture medium of Ralstonia sp. A-471 by precipitation with (NH4)2SO4 and column chromatography with DEAE-Toyopearl 650M and Sephacryl S-200. The purified enzyme wasExpand
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A novel goose-type lysozyme gene with chitinolytic activity from the moderately thermophilic bacterium Ralstonia sp. A-471: cloning, sequencing, and expression
In this study, we cloned the gene encoding goose-type (G-type) lysozyme with chitinase (Ra-ChiC) activity from Ralstonia sp. A-471 genomic DNA library. This is the first report of another type ofExpand
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Expression of a gene encoding chitinase (pCA 8 ORF) from Aeromonas sp. no. 10S-24 in Escherichia coli and enzyme characterization.
A gene encoding chitinase from Aeromonas sp. no. 10S-24 was expressed using pTrc99A in Escherichia coli JM 105 which yielded a 5-fold higher activity than when pUC19 was used. Three differentExpand
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Laxative Potential of The Konjac Flour (Amorphophallus muelleri Blume) in Treatment of Loperamide Induced Constipation on Sprague Dawley Rats
There is long history of konjac tubers being used as a cure for certain diseases in China and Japan. Konjac flour is prepared from konjac tubers and it contains high concentration of glucomannan.Expand
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KARAKTERISTIK KANDUNGAN KIMIA DAN DAYA CERNA TEMPE SORGUM COKLAT (Sorghum bicolor) [Characteristics of Chemical Content and Digestibility of Brown Sorghum Tempeh]
Brown sorghum (Sorghum bicolor) is rarely used for food product because its protein has lower digestibility compared to other cereals. One factor affecting lower sorghum protein digestibility is antiExpand
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ENZIM KITINASE DAN APLIKASI DI BIDANG INDUSTRI: KAJIAN PUSTAKA [IN PRESS JULI 2015]
Manan merupakan polisakarida yang banyak ditemukan di alam serta sumber yang murah untuk produksi manosa dan manooligosakarida. Hidrolisis manan dapat dilakukan secara enzimatis. Pemanfaatan enzimExpand
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MINUMAN PROBIOTIK DARI AIR KELAPA MUDA DENGAN STARTER BAKTERI ASAM LAKTAT Lactobacillus casei [IN PRESS JULI 2015]
Kelapa merupakan tanaman tropis yang berlimpah di Indonesia. Kandungan gizi air kelapa muda adalah protein 0.20%, karbohidrat 5.80%, vitamin 3.00%, dan mineral 4.00%. Kandungan gizi air kelapa mudaExpand
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Optimasi Produksi Tepung Porang dari Chip Porang Secara Mekanis dengan Metode Permukaan Respons
Porang Tuber (Amorphophallus oncophyllus) has high Glucomanan content which is very useful in food and non food industry as well as in medical industry. The only main problem in development of porangExpand
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A chitinase with high activity toward partially N-acetylated chitosan from a new, moderately thermophilic, chitin-degrading bacterium, Ralstonia sp. A-471
A moderately thermophilic bacterium, strain A-471, capable of degrading chitin was isolated from a composting system of chitin-containing waste. Analysis of the 16S rDNA sequence revealed that theExpand
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Identification of a potent Angiotensin-I converting enzyme inhibitory peptide from Black cumin seed hydrolysate using orthogonal bioassay-guided fractionations coupled with in silico screening
Abstract Black cumin (Nigella sativa) seed protein (BCSP) was individually hydrolyzed with pepsin, trypsin, and α-chymotrypsin. After ultrafiltration, the α-chymotrypsin hydrolysate (
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