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In vivo immobilization of enzymatically active polypeptides on the cell surface of Staphylococcus carnosus
TLDR
It is demonstrated that it is possible to immobilize normally soluble enzymes on the cell wall of S. carnosus without radically altering their catalytic activity by fusing them to a cell‐wall‐immobilization unit, consisting of a suitable cellwall‐spanning region and a standard cell‐ wall sorting signal.
C-terminal glycine-histidine tagging of the outer membrane protein Iga beta of Neisseria gonorrhoeae.
TLDR
It is demonstrated that the glycine-histidine tag does not interfere with the assembly of Iga beta in the outer membrane and that the translocator function of the modified Iga Beta is maintained.