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Self- and actin-templated assembly of Mammalian septins.
It is concluded that septin alone self-assemble into rings, that adaptor proteins recruit septins to actin bundles, and that sePTins help organize these bundles. Expand
Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
It is shown that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis and continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components. Expand
Interphase chromosomes undergo constrained diffusional motion in living cells
It is found that chromatin is free to undergo substantial Brownian motion, but that a given chromatin segment is confined to a subregion of the nucleus, which leads to a model for the regulation of chromosome interactions by nuclear architecture. Expand
Divergent Signals and Cytoskeletal Assemblies Regulate Self-Organizing Polarity in Neutrophils
Like neutrophilic leukocytes, differentiated HL-60 cells respond to chemoattractant by adopting a polarized morphology, with F-actin in a protruding pseudopod at the leading edge and contractileExpand
GFP tagging of budding yeast chromosomes reveals that protein–protein interactions can mediate sister chromatid cohesion
It is concluded that sister Chromatid separation in budding yeast can occur in the absence of microtubule-dependent forces, and that protein complexes that can bind two different DNA molecules are capable of holding sister chromatids together. Expand
In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition
We report a new method for in situ localization of DNA sequences that allows excellent preservation of nuclear and chromosomal ultrastructure and direct, in vivo observations. 256 direct repeats ofExpand
Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms
This analysis suggests that different active processes are involved in movement and localization of the chromosome and of the two plasmids during segregation. Expand
Dual recognition of CENP-A nucleosomes is required for centromere assembly
CENP-C and CENP-N recognize distinct structural elements of CENP-A nucleosomes, providing a foundation for the assembly of other centromere and kinetochore components.
Dynamics of centromeres during metaphase-anaphase transition in fission yeast: Dis1 is implicated in force balance in metaphase bipolar spindle.
The Centromere showed dynamic movements throughout phase 2 as it moved back and forth and was transiently split in two before its separation, suggesting that the centromere was positioned in a bioriented manner toward the poles at metaphase. Expand
CENP-C recruits M18BP1 to centromeres to promote CENP-A chromatin assembly
CENP-C provides a link between existing CENP-A chromatin and the proteins required for new CENP-A nucleosome assembly.