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Composition and Hierarchical Organisation of a Spider Silk
- A. Sponner, W. Vater, S. Monajembashi, E. Unger, F. Grosse, K. Weißhart
- Materials Science, MedicinePloS one
- 3 October 2007
The morphological defined structural elements in dragline silk of Nephila clavipes are linked to their biochemical composition and physicochemical properties to understand the composite nature of silk and its supra-molecular organisation.
Characterization of the protein components of Nephila clavipes dragline silk.
- A. Sponner, B. Schlott, F. Vollrath, E. Unger, F. Grosse, K. Weißhart
- 2 March 2005
Spider silk is predominantly composed of structural proteins called spider fibroins or spidroins, and polypeptides reactive against both Masp 1 and 2 specific antibodies were found to be expressed in the epithelia of the tail and different gland zones and accumulated in the gland secreted material.
Differential polymerization of the two main protein components of dragline silk during fibre spinning
The findings suggest that the role of spidroin 2 in the spinning process could be to facilitate the formation of fibrils and contribute directly to the elasticity of the silk.
Proline and processing of spider silks.
These relationships, in combination with protein sequence data, support the hypothesis that the proline-related motif, that is, GPGXX, may play a key role in silk and explain the interspecific variability of spider dragline silk.
The conserved C-termini contribute to the properties of spider silk fibroins.
Differences in polyphosphate accumulation and phosphate adsorption by Acinetobacter isolates from wastewater producing polyphosphate: AMP phosphotransferase
Conserved C-termini of Spidroins are secreted by the major ampullate glands and retained in the silk thread.
Polyclonal antibodies derived against fusion proteins containing the conserved C-terminal regions of both Spidroin 1 and 2 from the spider Nephila clavipes showed a reduction in their size under reducing conditions indicating the presence of disulfide bonds, suggesting crucial roles the C- termini play in the formation and/or structure of the corresponding silk filaments.
Containment of Extended Length Polymorphisms in Silk Proteins
It is shown that sequence variations expressed as deletions or insertions of tri-nucleotides lead to different sized and structured repetitive units throughout a silk protein, which suggests that a certain size range stabilized by purifying selection is important for spidroin 1 gene integrity and protein function.
Polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge.
The polyphosphate-dependent enzymes reported here may play an additional role in the complex process of 'biological' phosphate removal from wastewater, especially for anabolic processes.
Spider silk as a resource for future biotechnologies
- A. Sponner
- 1 December 2007
There is more to Arachne's weave and science is beginning to pick up those threads, and some dragline silks such as the major ampullate silks of some Nephila species show amazing mechanical properties that, in terms of toughness, are far superior to Bombyx silk.