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The Structure of the Elicitor Cerato-platanin (CP), the First Member of the CP Fungal Protein Family, Reveals a Double ψβ-Barrel Fold and Carbohydrate Binding*
TLDR
The results suggest that CP might be involved in polysaccharide recognition and that the double ψβ-barrel fold is widespread in distantly related organisms, where it is often involved in host-microbe interactions. Expand
Selective reciprocity in antimicrobial activity versus cytotoxicity of hBD-2 and crotamine
TLDR
The hypothesis that antimicrobial and cytotoxic polypeptides have ancestral structure-function homology, but evolved to preferentially target respective microbial versus mammalian ion channels via residue-specific interactions is supported. Expand
Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terrificus venom.
TLDR
This is the first report on the structure of a toxin from snake venom active on Na+ channel determined by proton NMR spectroscopy and it is suggested to exhibit analgesic activity and to be myonecrotic. Expand
Solution structure of a recombinant mouse major urinary protein.
TLDR
The refined high-resolution three-dimensional solution structure of rMUP in the native state is described, obtained by a combination of distance geometry and energy minimization calculations based on 2362 NOE-derived distance restraints. Expand
Spectroscopic evidence for a preferential location of lidocaine inside phospholipid bilayers.
TLDR
A "transient site" model for lidocaine preferential location in EPC bilayers is proposed, based on the consideration that insertion of the bulky aromatic ring of the anesthetic into the glycerol backbone region causes a decrease in the mobility of that EPC region and an increased mobility of the acyl chains. Expand
Structure of the C-terminal Fragment 300-320 of the Rat Angiotensin II AT1A Receptor and Its Relevance with Respect to G-Protein Coupling*
TLDR
It is suggested that the capability of the peptide to modulate its secondary structure as a function of the medium dielectric constant, as well as its ability to form helical aggregates by means of intermolecular hydrophobic interactions, can play a significant role for G-protein activation. Expand
1 THE STRUCTURE OF THE ELICITOR CERATO-PLATANIN , FOUNDER OF THE CP FUNGAL PROTEIN FAMILY , REVEALS A DOUBLE Ψβ-BARREL FOLD AND CARBOHYDRATE BINDING
*Correspondence to: Alberto Spisni, Università degli Studi di Parma, Via Volturno, 39, 43125, Parma, Italy, Tel. +39 0521033807; Fax +39 0521033802. E-Mail: alberto.spisni@unipr.it ; Daniel O.Expand
How C-terminal carboxyamidation alters the biological activity of peptides from the venom of the eumenine solitary wasp.
TLDR
The diverse biological activity of the two peptides is not simply due to the reduction of the net positive charge, as generally suggested, but also to a structural perturbation of the amphipathic alpha-helix that affects their ability to perturb the cell membrane. Expand
Model peptides mimic the structure and function of the N-terminus of the pore-forming toxin sticholysin II.
To investigate the role of the N-terminal region in the lytic mechanism of the pore-forming toxin sticholysin II (St II), we studied the conformational and functional properties of peptidesExpand
Expression of a lipocalin in Pichia pastoris: secretion, purification and binding activity of a recombinant mouse major urinary protein
TLDR
Binding activity, tested with an odorant molecule which displays high affinity for native MUP, indicates that rMUP has a behavior similar to the native one, and suggests that the protein, and in particular its hydrophobic binding pocket, is properly folded. Expand
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