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Characterization of Native and Recombinant Falcipain-2, a Principal Trophozoite Cysteine Protease and Essential Hemoglobinase ofPlasmodium falciparum *
The results suggest thatfalcipain-2 can initiate cleavage of native hemoglobin in the P. falciparum food vacuole, that, following initial cleavages, the protease plays a key role in rapidly hydrolyzing globin fragments, and that a drug discovery effort targeted at this protease is appropriate. Expand
Expression and characterization of the Plasmodium falciparum haemoglobinase falcipain-3.
Falcipain-3 is a second P. falciparum haemoglobinase that is particularly suited for the hydrolysis of native haemochemistry in the acidic food vacuole, and may offer optimized hydroolysis of both native ha Hemoglobin and globin peptides. Expand
Polymorphisms in the Plasmodium falciparum pfcrt and pfmdr-1 genes and clinical response to chloroquine in Kampala, Uganda.
In evaluations of known polymorphisms in parasites from patients with uncomplicated malaria in Kampala, Uganda, the presence of 8 pfcrt mutations and 2 pfmdr-1 mutations did not correlate with clinical response to therapy with chloroquine, suggesting that other factors may contribute to clinical outcomes. Expand
Preferential coreceptor utilization and cytopathicity by dual-tropic HIV-1 in human lymphoid tissue ex vivo.
HIV-1 coreceptor usage in vitro cannot be unconditionally extrapolated to natural microenvironment of human lymphoid tissue, and dual-tropic viruses are probably a continuum between the 2 polar variants that use CXCR4 or CCR5 exclusively; and cytopathicity toward the general CD4(+) T cell population in lymphoids tissue is associated with the use of CX CR4. Expand
Characterization of large plasmids encoding resistance to toxic heavy metals in Salmonella abortus equi.
It is established for the first time the transferable large plasmids encoding resistance to various heavy metals, antibiotics and biochemical nature of S. abortus equi. Expand
Mutation of the non-Mendelian suppressor, Psi, in yeast by hypertonic media.
The results suggest that the replication of the Psi(+) determinant is sensitive to hypertonic conditions, and may be affected by some component of the translational machinery. Expand
Nonsense suppressors of yeast cause osmotic-sensitive growth.
  • A. Singh
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1977
This heretofore unknown property of nonsense suppressor is useful for mitotic mapping, selecting tRNA mutants, selecting antisuppressors, and scoring nonsense suppressors. Expand
Functional characterization of the Aspergillus nidulans glucosylceramide pathway reveals that LCB Δ8‐desaturation and C9‐methylation are relevant to filamentous growth, lipid raft localization and
C8‐desaturated and C9‐methylated glucosylceramide (GlcCer) is a fungal‐specific sphingolipid that plays an important role in the growth and virulence of many species. In this work, we investigatedExpand
Saccharomyces cerevisiae contains two discrete genes coding for the alpha-factor pheromone.
The alpha-factor can be derived from 2 different precursor proteins of 165 and 120 amino acids containing, respectively, 4 and 2 copies of the pheromone. Expand
Aspergillus nidulans alpha-1,3 glucanase (mutanase), mutA, is expressed during sexual development and mobilizes mutan.
Results suggest that additional carbon sources are available during sexual development in Aspergillus nidulans, including alpha-1,3 glucan, which is considered the main reserve material accumulated during vegetative growth as a cell wall component and consumed duringSexual development. Expand