Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
- D. Klionsky, H. Abeliovich, R. Deter
- BiologyAutophagy
- 16 February 2008
A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented.
EEA1 links PI(3)K function to Rab5 regulation of endosome fusion
- A. Simonsen, R. Lippé, H. Stenmark
- Biology, Computer ScienceNature
- 30 July 1998
The identification of EEA1 as a direct Rab5 effector provides a molecular link between PI(3)K and Rab5, and its restricted distribution to early endosomes indicates that EEA 1 may confer directionality to Rab5-dependent endocytic transport.
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
- D. Klionsky, K. Abdelmohsen, S. Zughaier
- BiologyAutophagy
- 18 April 2012
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
Molecular definitions of autophagy and related processes
- L. Galluzzi, E. Baehrecke, G. Kroemer
- BiologyEMBO Journal
- 3 July 2017
A panel of leading experts in the field attempts here to define several autophagy‐related terms based on specific biochemical features to formulate recommendations that facilitate the dissemination of knowledge within and outside the field of autophagic research.
Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease
- M. Filimonenko, Susanne Stuffers, A. Simonsen
- BiologyJournal of Cell Biology
- 5 November 2007
It is shown that autophagic degradation is inhibited in cells depleted of ESCRT subunits and in cells expressing CHMP2B mutants, leading to accumulation of protein aggregates containing ubiquitinated proteins, p62 and Alfy, and functional MVBs are required for clearance of TDP-43 and expanded polyglutamine aggregates associated with Huntington's disease.
FYVE fingers bind PtdIns(3)P
- J. Gaullier, A. Simonsen, A. D’Arrigo, B. Bremnes, H. Stenmark, R. Aasland
- BiologyNature
- 30 July 1998
The FYVE finger, an evolutionarily conserved double-zinc-binding domain, is identified as a protein structure that binds to PtdIns(3)P with high specificity.
The role of phosphoinositides in membrane transport.
- A. Simonsen, A. Wurmser, S. Emr, H. Stenmark
- Biology, ChemistryCurrent Opinion in Cell Biology
- 1 August 2001
Coordination of membrane events during autophagy by multiple class III PI3-kinase complexes
- A. Simonsen, S. Tooze
- BiologyJournal of Cell Biology
- 21 September 2009
The cross talk between autophagy and intracellular compartments is discussed, highlighting recent exciting data about the role and regulation of the Vps34 class III phosphatidylinositol (PI) 3-kinase in autophophagy.
Promoting basal levels of autophagy in the nervous system enhances longevity and oxidant resistance in adult Drosophila
- A. Simonsen, R. Cumming, A. Brech, P. Isakson, D. Schubert, K. Finley
- BiologyAutophagy
- 16 February 2008
It is demonstrated that expression of several autophagy genes is reduced in Drosophila neural tissues as a normal part of aging, indicating that genetic or age-dependent suppression ofAutophagy is closely associated with the buildup of cellular damage in neurons and a reduced lifespan, while maintaining the expression of a rate-limiting Autophagy gene prevents the age- dependent accumulation of damage in neuron and promotes longevity.
Autophagy in malignant transformation and cancer progression
- L. Galluzzi, F. Pietrocola, G. Kroemer
- Biology, MedicineEMBO Journal
- 1 April 2015
The differential impact of autophagy on distinct phases of tumorigenesis is discussed and the implications of this concept for the use of Autophagy modulators in cancer therapy are discussed.
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