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Nested expression domains of four homeobox genes in developing rostral brain
The expression domains of the four genes in the developing rostral brain of mouse embryos at a developmental stage, day 10 post coitum, seem to be continuous regions contained within each other in the sequence Emxl < Emx2 < Otxl< Otx2.
A vertebrate gene related to orthodenticle contains a homeodomain of the bicoid class and demarcates anterior neuroectoderm in the gastrulating mouse embryo.
The expression patterns of the two genes in diencephalon suggest that they both have a role in establishing the boundary between presumptive dorsal and ventral thalamus and in anterior neuroectoderm, demarcating rostral brain regions even before headfold formation.
Reorganization of enhancer patterns in transition from naive to primed pluripotency.
Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation.
It is suggested that Otx2 expression in endomesoderm and ectoderm is required for anterior neuroectoderm specification, and in gastrulating heterozygous embryos, a post-transcriptional repression acts on lacZ transcripts in the ectodermal layer, but not in the external layer, suggesting that different post- transcriptional mechanisms control Otx1 expression in both layers.
Two vertebrate homeobox genes related to the Drosophila empty spiracles gene are expressed in the embryonic cerebral cortex.
- A. Simeone, M. Gulisano, D. Acampora, A. Stornaiuolo, M. Rambaldi, E. Boncinelli
- BiologyThe EMBO journal
- 1 July 1992
We cloned two homeobox genes, Emx1 and Emx2, related to empty spiracles, a gene expressed in very anterior body regions during early Drosophila embryogenesis, and studied their expression in mouse…
Craniofacial, vestibular and bone defects in mice lacking the Distal-less-related gene Dlx5.
The defects observed in Dlx5-/- mutant animals suggest multiple and independent roles of this gene in the patterning of the branchial arches, in the morphogenesis of the vestibular organ and in osteoblast differentiation.
Otx1 and Otx2 activities are required for the normal development of the mouse inner ear.
The results suggest that both Otx genes play important and differing roles in the morphogenesis of the mouse inner ear and the development of its sensory organs.
Otx genes are required for tissue specification in the developing eye.
A model is proposed in which Otx gene products are required for the determination and differentiation of the pigment epithelium, co-operating with other eye patterning genes in the determination of the specialised tissues that will constitute the mature vertebrate eye.
The caudal limit of Otx2 gene expression as a marker of the midbrain/hindbrain boundary: a study using in situ hybridisation and chick/quail homotopic grafts.
Long-survival analysis allowing the recognition of the various grisea of the chimeric brains strongly supports the view that, as early as stage HH10, the caudal limit of Otx2 expression separates mesencephalic from isthmo/cerebellar territories.
Cloning and characterization of two members of the vertebrate Dlx gene family.
- A. Simeone, D. Acampora, K. Huebner
- BiologyProceedings of the National Academy of Sciences…
- 15 March 1994
The expression pattern of these genes, together with their chromosome localization, may provide useful cues for the study of congenital disorders in which there is a combination of craniofacial and limb defects.