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The CATH Domain Structure Database and related resources Gene3D and DHS provide comprehensive domain family information for genome analysis
The CATH database of protein domain structures currently contains 43 229 domains classified into 1467 superfamilies and 5107 sequence families is expanded with sequence relatives from GenBank and completed genomes, using a variety of efficient sequence search protocols and reliable thresholds. Expand
Protein Superfamily Evolution and the Last Universal Common Ancestor (LUCA)
Functional analysis of a set of 140 ancestral protein domains reveals a genetically complex LUCA with practically all the essential functional systems present in extant organisms, supporting the theory that life achieved its modern cellular status much before the main kingdom separation. Expand
Hydrolysis of bis(5'-nucleosidyl) polyphosphates by Escherichia coli 5'-nucleotidase
A different enzyme activity that hydrolyzes dinucleoside polyphosphates is described, which was purified from sonic extracts and osmotic shock fluid and was able to split diadenosine triphosphate and adenosine-(5')-diphospho-(5)-beta-D-ribose, ribose-5-ph phosphate, and dinucleose polyph phosphates. Expand
Isoelectric points of proteins: theoretical determination.
Three methods for calculating the isoelectric points (pI) of proteins, provided that their amino acid compositions are known, are described. The comprehensive and abridged procedures involveExpand
In Saccharomyces cerevisiae, the effect of H2O2 on ATP, but not on glyceraldehyde-3-phosphate dehydrogenase, depends on the glucose concentration
As has been previously shown, Saccharomyces cerevisiae grown in 2% or 0.025% glucose uses this carbohydrate by the fermentative or oxidative pathways, respectively, and the effect of the addition of H2O2 on the level of ATP and GAPDH activity differed. Expand
Enzymes involved in fructose metabolism in lir and the glyceraldehyde metabolic crossroads.
quantitative model of the metabolic crossroads of D-glyceraldehyde has been developed. This model permits a prediction of the relative contributions of the phosphorylative, oxidative and reductiveExpand
Dinucleosidetriphosphatase from Rat Liver
The enzyme requires Mg2+ or Mn2+, is maximally active at a pH value of approximately 7.5, and has a molecular weight of 29800 as estimated by filtration on Sephadex G-75. Expand
Wide occurrence of guanosine 5'-tetraphosphate in commercial preparations of GTP.
Abstract Guanosine 5′-tetraphosphate is present in commercial preparations of GTP in proportions from 1–4.5%, apparently regardless of the type of salt, method of preparation, age of the sample orExpand
Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase and several ligases.
The findings presented here originally arose from the suggestion that the synthesis of dinucleoside polyphosphates (Np(n)N) may be a general process involving enzyme ligases catalyzing the transferExpand