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Wood frog adaptations to overwintering in Alaska: new limits to freezing tolerance
It is hypothesize that this enhancement of freeze tolerance in Alaskan wood frogs is due to higher cryoprotectant levels that are produced by repeated freezing and thawing cycles experienced under natural conditions during early autumn. Expand
Modification of Proteins In Vitro by Physiological Levels of Glucose
Investigation of the conversion of protein-Amadori intermediate to protein-AGE and the mechanism of its inhibition by pyridoxamine, a potent AGE inhibitor that has been shown to prevent diabetic complications in animal models demonstrated that PM does not react with the Amadori. Expand
Hydroxymethyl group conformation in saccharides: structural dependencies of (2)J(HH), (3)J(HH), and (1)J(CH) spin-spin coupling constants.
New Karplus equations derived from J-couplings computed from density functional theory (DFT) in a model aldopyranosyl ring are in excellent agreement with experimental values and with couplings predicted from a previously reported general Karplus equation, confirming the reliability of DFT-calculated (1]H-(1)H couplings in saccharides. Expand
Carbon-13-enriched carbohydrates: preparation of triose, tetrose, and pentose phosphates.
Three-, four-, and five-carbon aldononitrile phosphates were prepared, purified, and catalyticlly reduced with palladium--barium sulfate (5%) to the corresponding aldose phosphates in high yields atExpand
A nonprotein thermal hysteresis-producing xylomannan antifreeze in the freeze-tolerant Alaskan beetle Upis ceramboides
This xylomannan is the first TH-producing antifreeze isolated from a freeze-tolerant animal and the first in a new class of highly active THFs that contain little or no protein. Expand
Pyridoxamine protects proteins from functional damage by 3-deoxyglucosone: mechanism of action of pyridoxamine.
It is demonstrated that the important physiological RCS 3-deoxyglucosone (3-DG) can damage protein functionality, including the ability of collagen IV to interact with glomerular mesangial cells, and PM can protect against 3-DDG-induced protein damage via a novel mechanism. Expand
A thermal hysteresis-producing xylomannan glycolipid antifreeze associated with cold tolerance is found in diverse taxa
It appears that antifreeze glycolipids play a role in cold tolerance, as 1H NMR spectra of the ice-purified molecules isolated from these diverse freeze-tolerant and freeze-avoiding organisms were nearly identical, indicating that the chemical structures of the glycolIPids were highly similar. Expand
The role of endogenous antifreeze protein enhancers in the hemolymph thermal hysteresis activity of the beetle Dendroides canadensis.
Hemolymph AFPs were not maximally enhanced since addition of the potent enhancer citrate (at non-physiologically high levels) resulted in large increases in THA, and NMR showed that glycerol was the only low molecular mass solute present in sufficiently high concentrations in the hemolymph to function as an enhancer. Expand