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Engineering Escherichia coli for the synthesis of taxadiene, a key intermediate in the biosynthesis of taxol.
TLDR
The success of both in vitro and in vivo synthesis of taxadiene bodes well for the future production of taxoids by non-paclitaxel producing organisms through pathway engineering. Expand
Interpretation of the ultraviolet spectra of natural products
Interpretation of the ultraviolet spectra of natural products , Interpretation of the ultraviolet spectra of natural products , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
Structure of the Methanococcus jannaschii Mevalonate Kinase, a Member of the GHMP Kinase Superfamily*
TLDR
Analysis of the MVK model in the context of the other members of the GHMP kinase family offers the opportunity to understand both the mechanism of these enzymes and the structural details that may lead to the design of novel drugs. Expand
Sterol carrier protein-2: structure reveals function
TLDR
Comparison of the apo- and holoprotein amide fingerprints revealed about 60% of the resonances remaining essentially unchanged, 12 assigned amide residues underwent significant chemical-shift changes upon oleic acid binding, and circular dichroism showed that these chemical- shift changes did not alter the secondary structure of SCP-2. Expand
Identification and Characterization of a Novel Vitamin B12 (Cobalamin) Biosynthetic Enzyme (CobZ) from Rhodobacter capsulatus, Containing Flavin, Heme, and Fe-S Cofactors*
TLDR
Recombinant overproduction of Orf663, now renamed CobZ, allowed the characterization of a novel cofactor-rich protein, housing two Fe-S centers, a flavin, and a heme group, which like B12 itself is a modified tetrapyrrole. Expand
Overcoming product inhibition of S-Adenosyl-L-methionine (SAM) synthetase: Preparation of SAM on the 30 mM scale
Abstract Product inhibition of SAM synthetase could be overcome in cell free incubations using up to 30 mM methionine in the presence of salts of organic acids such as sodium p -toluenesulfonate ( pExpand
13 C n.m.r. evidence for a new intermediate, pre-uroporphyrinogen, in the enzymic transformation of porphobilinogen into uroporphyrinogens I and III
Evidence is presented from 13C n.m.r. spectroscopic studies which indicates that the enzymic transformation of porphobilinogen into uroporphyrinogens I and III occurs through a transient freeExpand
Tetrapyrrole assembly and modification into the ligands of biologically functional cofactors.
TLDR
It is discussed how the oxidation level and chromophoric arrangement of the uroporphinoid ring, rather than its substitution pattern, provides the necessary molecular recognition for some of the later enzymes, whose function is to decorate the template by C-methylation on the way to the biologically active cofactors. Expand
Enzymatic synthesis of S-adenosyl-L-methionine on the preparative scale.
TLDR
It is demonstrated that product inhibition of the E. coli SAM synthetase can also be overcome by adding a high concentration of beta-mercaptoethanol, acetonitrile, or urea. Expand
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