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Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase
A key limitation of whole‐cell redox biocatalysis for the production of valuable, specifically functionalized products is substrate/product toxicity, which can potentially be overcome by usingExpand
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Characterization and Application of Xylene Monooxygenase for Multistep Biocatalysis
ABSTRACT Xylene monooxygenase of Pseudomonas putida mt-2 catalyzes multistep oxidations of one methyl group of toluene and xylenes. Recombinant Escherichia coli expressing the monooxygenase genesExpand
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TADH, the thermostable alcohol dehydrogenase from Thermus sp. ATN1: a versatile new biocatalyst for organic synthesis
The alcohol dehydrogenase from Thermus sp. ATN1 (TADH) was characterized biochemically with respect to its potential as a biocatalyst for organic synthesis. TADH is a NAD(H)-dependent enzyme andExpand
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Quantification of metabolic limitations during recombinant protein production in Escherichia coli.
Escherichia coli is one of the major microorganisms for recombinant protein production because it has been best characterized in terms of molecular genetics and physiology, and because of theExpand
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Simple enzymatic procedure for l‐carnosine synthesis: whole‐cell biocatalysis and efficient biocatalyst recycling
β‐Peptides and their derivates are usually stable to proteolysis and have an increased half‐life compared with α‐peptides. Recently, β‐aminopeptidases were described as a new enzyme class thatExpand
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Characterization of the C-terminal ER membrane anchor of PTP1B.
The tyrosine phosphatase PTP1B is an important regulator of cell function. In living cells PTP1B activity is restricted to the vicinity of the endoplasmic reticulum (ER) by post-translationalExpand
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Isolated Microbial Single Cells and Resulting Micropopulations Grow Faster in Controlled Environments
ABSTRACT Singularized cells of Pichia pastoris, Hansenula polymorpha, and Corynebacterium glutamicum displayed specific growth rates under chemically and physically constant conditions that wereExpand
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An artificial TCA cycle selects for efficient α‐ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli
Amino acid hydroxylases depend directly on the cellular TCA cycle via their cosubstrate α‐ketoglutarate (α‐KG) and are highly useful for the selective biocatalytic oxyfunctionalization of aminoExpand
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Engineering of Pseudomonas taiwanensis VLB120 for Constitutive Solvent Tolerance and Increased Specific Styrene Epoxidation Activity
ABSTRACT The application of whole cells as biocatalysts is often limited by the toxicity of organic solvents, which constitute interesting substrates/products or can be used as a second phase for inExpand
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Novel cyclohexane monooxygenase from Acidovorax sp. CHX100
Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. TransposonExpand
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