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Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae.
TLDR
This finding, together with the apparent zymogenic nature of Chs2, is consistent with the hypothesis, previously put forward for Chs1, that localized deposition of chitin is attained by activation of the zymogen form at a specific time and place. Expand
Chitin synthase 3 from yeast has zymogenic properties that depend on both the CAL1 and the CAL3 genes.
TLDR
It has been found that the substrate UDPGlcNAc protects chitin synthase 3 against proteolytic inactivation and is concluded that Chs3 is a zymogen and that the CAL2 product functions as its activator. Expand
Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes.
TLDR
Inverse metabolic engineering means the elucidation of a metabolic engineering strategy by determining the genetic or the particular environmental factors conferring that phenotype and endowing that phenotype on another strain or organism by directed genetic or environmental manipulation. Expand
The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo
TLDR
Three CHS1 gene disruption experiments were performed, demonstrating that strains with the disrupted gene have a recognizable phenotype, lack measurable chitin synthase activity in vitro but are viable, contain normal levels of chitIn in vivo, and mate and sporulate efficiently. Expand
Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae
TLDR
It is concluded that damage to the cell wall is caused by excessive chitinase activity at acidic pH, which can normally be repaired through chit in synthesis by Chs1, and the latter emerges as an auxiliary or emergency enzyme. Expand
Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae.
TLDR
The mechanism of in vivo synthesis of chitin has been clarified by cloning the structural gene for the newly found chitIn synthase 2, a relatively minor activity in yeast, establishing that the gene product is essential for both septum formation and cell division. Expand
Prothymosin alpha antisense oligomers inhibit myeloma cell division.
TLDR
It is concluded that antisense DNA caused specific hybrid arrest of translation in prothymosin alpha that is required for cell division, and there is no evidence that prothysosinalpha directly regulates mitosis. Expand
Nuclear targeting of prothymosin alpha.
TLDR
The basic cluster of amino acids at the carboxyl terminus of prothymosin alpha, TKKQKT, has been identified as part of the nuclear targeting signal, whereas the basic clusterof amino acids situated within the thymos in alpha 1 sequence at the amino terminus failed to effect nuclear transport. Expand
Fungal cell wall synthesis: the construction of a biological structure.
TLDR
Regulatory mechanisms that act on chitin and beta(1----3) glucan synthetase indicate how localized biosynthesis can be achieved in the fungal cell. Expand
Engineering of coordinated up- and down-regulation of two glycosyltransferases of the O-glycosylation pathway in Chinese hamster ovary (CHO) cells.
TLDR
Partial cloning of the CMP-sialic, acid:Galbeta1,3GalNAcalpha2,3-Sialyltransferase (ST3Gal I) gene from Chinese hamster ovary cells and the simultaneous inhibition and activation of the two key enzyme activities in the O-glycosylation pathway of mammalian cells are reported, an important addition to the metabolic engineering field. Expand
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