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Comparison between RT-PCR, NASBA and RT-LAMP Methods for Detection of Coxsackievirus B3
TLDR
Based on the results that obtained in this study, the RT-LAMP assay was highest sensitive than RT-PCR and NASBA techniques for detection of CVB3 infection.
Intein-Mediated Fusion Soluble Expression of Recombinant Human Interferon Beta (rhIFN-β)
TLDR
The expression of IFNβ fused with INT and CBD can be a suitable strategy for the soluble expression of this pharmaceutical protein and the auto-cleavage characteristic of INT induced by thiol agent, temperature or pH can be used to increase the protein solubilization in the process of producing IFN β protein.
Design of Localized Surface Plasmon Resonance (LSPR) Biosensor for Immunodiagnostic of E. coli O157:H7 Using Gold Nanoparticles Conjugated to the Chicken Antibody
TLDR
A novel nano-bio probe constructed by synthesizing citrate gold nanoparticle conjugated with specific chicken anti-E.
Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris
TLDR
Human granulocyte-colony stimulating factor cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter to obtain high cell density and higher levels of protein expression.
Design and Characterization of an Aequorin‐based Bacterial Biosensor for Detection of Toluene and Related Compounds
TLDR
An aequorin‐based Escherichia coli strain JM109 biosensor was constructed and characterized for its potential to detect toluene and related compounds in aqueous solutions and it is shown that this reporter enzyme has reasonable sensitivity compared with other reporter systems.
Stabilisation of Recombinant Aequorin by Polyols: Activity, Thermostability and Limited Proteolysis
TLDR
Sorbitol increases stability of aequorin with high photoactivity and not effect for flexibility and limited proteolysis of this photoprotein.
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