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TAXONOMIC REVISION OF THE FAMILY CLOSTEROVIRIDAE WITH SPECIAL REFERENCE TO THE GRAPEVINE LEAFROLL-ASSOCIATED MEMBERS OF THE GENUS AMPELOVIRUS AND THE PUTATIVE SPECIES UNASSIGNED TO THE FAMILY
TLDR
A comparative examination of the amino acid sequence divergence of three taxonomically relevant genes supports the notion that they are all genetically divergent variants of a single species, GLRaV-4, and the establishment of a fourth genus within the family Closteroviridae is justified.
Deep sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that includes a novel virus.
TLDR
An analysis of total plant RNA sequences using Life Sciences 454 high-throughput sequencing revealed that a vine showing decline symptoms supported a mixed infection that included seven different RNA genomes.
Quasispecies nature of the genome of Grapevine fanleaf virus.
TLDR
The data suggest that the GFLV genome consists of quasispecies populations, and that the subcloned sequences were found to vary from each other by as much as 13%, and the encoded amino acid sequences by as little as 9%.
Grapevine leafroll-associated virus 3
TLDR
The characterization of sgRNAs produced during the infection cycle of GLRaV-3 has given some insight into the replication strategy and the putative functionality of the ORFs, and the application of next-generation sequencing is proving to be a valuable tool to study the complexity of viral infection as well as plant pathogen interaction.
Real-time RT-PCR (TaqMan) assays for the detection of Grapevine Leafroll associated viruses 1-5 and 9.
TLDR
TaqMan RT-PCR was more sensitive than conventional one-step RT- PCR for testing different isolates of these viruses either using RNA or crude tissue extract and showed that when using total RNA extracted either by the Qiagen RNeasy method or by an ABI automated system more isolates were detected.
Nucleotide sequence and rt-PCR detection of a virus associated with grapevine rupestris stem-pitting disease.
TLDR
Sequence analysis indicated that the virus is most similar to apple stem-pitting virus and may be allied with the carla- and potexviruses and grouped with other viruses that infect woody hosts.
Application of a spotting sample preparation technique for the detection of pathogens in woody plants by RT-PCR and real-time PCR (TaqMan).
TLDR
The sample preparation techniques reported here could be used to store samples for future PCR test or for long distance shipment to a detection laboratory.
Grapevine leafroll-associated virus 1 occurs as genetically diverse populations.
TLDR
The genetic landscape of GLRaV-1 populations presented in this study provides a foundation for better understanding of the epidemiology of grapevine leafroll disease across grape-growing regions in the United States.
Molecular analysis of a California strain of Rupestris stem pitting-associated virus isolated from declining Syrah grapevines
TLDR
The sequence of the genome of a Rupestris stem pitting-associated virus isolated from a declining Syrah grapevine in California, designated the Syrah strain (RSPaV-SY), was determined and the coat protein was the most conserved gene among R SPaV sequences and the replicase was the least conserved genes.
Pathogen testing and certification of Vitis and Prunus species.
TLDR
A strong case is presented for the continued use of slower biological tests in programs requiring high levels of confidence in detection of pathogens that must be excluded from valuable germplasm.
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