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DNA markers for identifying biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) and studying population dynamics
Analysis of field samples collected in Israel for several years, using DNA markers developed, applied and compared, indicated that the percentage of the Q biotype tends to increase in field populations as time progresses, which may be attributed to the resistance to neonicotinoids and pyriproxyfen and the susceptibility of the B biotype to these insecticides.
Improved detection of prunus necrotic ringspot virus by the polymerase chain reaction
The RT-PCR assay described in this paper is sensitive enough for detection of PNRSV in dormant woody bark tissue and could be incorporated into testing protocols during post-entry quarantines for rapid initial screening of imported budwood and in virus elimination programs.
A zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on multiplication.
Aphid transmissibility was restored in a progeny virus derived from an infectious clone of the ZYMV-NAT isolate in which Thr was changed back to Ala by site-directed mutagenesis, but this mutation did not have any effect on the multiplication rate in squash, which was significantly higher than that of the AT isolate.
Infectious in vitro RNA transcripts derived from cloned cDNA of the cucurbit potyvirus, zucchini yellow mosaic virus.
The authenticity of the progeny virus was verified by identifying a specific molecular marker (C substituted for T in the 3' non-coding region) using nucleotide sequence analysis.
A New Pathotype of Pepper mild mottle virus (PMMoV) Overcomes the L4 Resistance Genotype of Pepper Cultivars.
The biological, serological, and molecular characteristics of a newly isolated L4 resistance-breaking isolate of Pepper mild mottle virus were studied and the new pathotype was designated P1,2,3,4 of PMMoV-Is.
Truncated Rep gene originated from Tomato yellow leaf curl virus-Israel [Mild] confers strain-specific resistance in transgenic tomato
Transgenic tomato plants carrying a truncated replication associated protein (T-Rep) gene of the mild strain of Tomato yellow leaf curl virus-Israel (TYLCV-Is [Mild]) were prepared, but these plants were susceptible to the severe strain of TYLCv-Is.
The use of the polymerase chain reaction (PCR) for the detection of bean yellow mosaic virus in gladiolus.
Combining PCR with molecular hybridisation (using a 32P-labelled transcript of viral sequences as a probe), further increased the sensitivity of this method to a gain of four to five orders of magnitude as compared with direct molecular hybrids, and enabled the detection of up to single picogram quantities of the virus.