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A comparative approach to determining the role of fish predation in structuring limnetic ecosystems
Results show that large differences between the lakes in the biomasses and body size of components at the top of the food web are diminished at lower trophic levels, suggesting that this attrition in the impacts of top-down control is due to compensatory mechanisms of prey populations.
Replication timing of two human common fragile sites: FRA1H and FRA2G
The results indicate that the fragile sequences are slow replicating and that they enter G2 phase unreplicated with very high frequency, which means these regions could sometimes reach mitosis unreplicate or undercondensed and be expressed as chromosome gaps/breakages.
Organization, nucleotide sequence, and chromosomal mapping of a tandemly repeated unit containing the four core histone genes and a 5S rRNA gene in an isopod crustacean species.
A tandemly repeated unit of 6553 bp containing a copy of the four core histone genes H2B, H2A, H3, and H4, and also a 5S rRNA gene, was amplified by PCR from genomic DNA of the isopod crustacean Asellus aquaticus to locate the gene cluster on an inter-individually variable number of chromosomes.
Characterization of the human common fragile site FRA2G.
5S ribosomal and U1 small nuclear RNA genes: a new linkage type in the genome of a crustacean that has three different tandemly repeated units containing 5S ribosomal DNA sequences.
Three different tandemly repeated units containing 5S rDNA were identified using PCR amplification and used as probes in fluorescent in situ hybridization (FISH) experiments to locate them on chromosomes of A. aquaticus.
Effects of distamycin A on human leukocytes in vitro.
Distamycin A, an oligopeptide antibiotic, supplied at various concentrations for 24 h to human leukocytes in culture, has induced the appearance on some chromosomes of specific areas lacking…
Effects of DAPI on human leukocytes in vitro.
DAPI (4'-6-diamidino-2-phenylindole), a fluorochrome specific for AT-rich DNA, was supplied for 24 h at various concentrations to human leukocytes in culture. This treatment caused the appearance on…
Breakages at common fragile sites set boundaries of amplified regions in two leukemia cell lines K562 - Molecular characterization of FRA2H and localization of a new CFS FRA2S.
On the Heterogeneity of Heterochromatin
- A. Rocchi
The use of staining techniques or in vivo treatments makes it possible to demonstrate the existence of a marked heterogeneity of fractions of heterochromatin sometimes located on the same chromosome or even belonging to the same heterochromaatin block.
Sex chromosome differentiation revealed by genomic in-situ hybridization
GISH did not reveal any sex chromosome molecular differentiation on the male and female homomorphic sex chromosome pair, and the karyotypes of these individuals were equally labelled by the male- and female-derived probe, while the heteromorphic Y chromosome showed a differentially labelled region only with themale- derived probe.