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FTO controls reversible m6Am RNA methylation during snRNA biogenesis
TLDR
The data reveal that FTO controls a previously unknown central step of snRNA processing involving reversible methylation, and suggest that epitranscriptomic information in snRNA may influence mRNA splicing. Expand
GGA1 Is Expressed in the Human Brain and Affects the Generation of Amyloid β-Peptide
TLDR
The data indicate that changes in the subcellular trafficking of BACE1 or other GGA1-dependent proteins contribute to changes in APP processing and Aβ generation, and GGA proteins might be involved in the pathogenesis of AD. Expand
Conformational changes in the expression domain of the Escherichia coli thiM riboswitch
TLDR
The secondary structure of the entire thiM riboswitch from Escherichia coli in its TPP-free form and its transition into the TPP-bound variant is reported, thereby depicting domains of the Riboswitch that serve as communication links between the aptamer and the expression domain. Expand
A diverse set of family 48 bacterial glycoside hydrolase cellulases created by structure‐guided recombination
TLDR
This work recombined the catalytic domains of three glycoside hydrolase family 48 bacterial cellulases to create a diverse library of Cel48 enzymes with an average of 106 mutations from the closest native enzyme, and correctly predicted sequences that were as stable as any of the native Cel48 enzyme described to date. Expand
Structural Elucidation of the Bispecificity of A Domains as a Basis for Activating Non-natural Amino Acids.
TLDR
The recently discovered A domain of an Anabaenopeptin synthetase from Planktothrix agardhii (ApnA A1) is capable of activating two chemically distinct amino acids (Arg and Tyr). Expand
RNA aptamers selectively modulate protein recruitment to the cytoplasmic domain of beta-secretase BACE1 in vitro.
TLDR
The isolation and characterization of RNA aptamers that selectively target the B1-CT are described, showing that these RNAs bind to authentic BACE1 and providing evidence that the binding site is restricted to the membrane-proximal half of the C terminus. Expand
Bioorthogonal site-specific labeling of the 5'-cap structure in eukaryotic mRNAs.
TLDR
A chemo-enzymatic approach for site-specific labeling of 5'-capped RNAs based on bioorthogonal chemistry is presented and further modified using strain-promoted azide-alkyne cycloaddition (SPAAC). Expand
Synthetic mRNA capping
TLDR
The preparation and properties of mRNAs with non-natural caps providing novel features such as improved stability or enhanced translational efficiency and state-of-the art methods are discussed. Expand
An aptamer targeting the apical-loop domain modulates pri-miRNA processing.
TLDR
The results show that aptamers can be applied as agents that modulate pri-miRNA processing and as tools for elucidating mechanisms of this process, and the ability to modulate the maturation of miRNA by targeting the apical-loop domain supports the importance of these domains during pri- miRNA processing. Expand
Current Approaches for RNA Labeling in Vitro and in Cells Based on Click Reactions
TLDR
This minireview focuses on labeling strategies for RNA that rely on the click reaction, and highlights click reactions that have been used successfully to label modified RNA, and describes different strategies to introduce the required reactive groups into target RNA. Expand
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