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Transgalactosylation by thermostable β-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus
The hyperthermostable I²â€glycosidases from the Archaea Sulfolobus solfataricus (SsI²Gly) and Pyrococcus furiosus (CelB) hydrolyse I²â€glycosides of d‐glucose or d‐galactose with relaxedExpand
Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose: II. Oligosaccharide formation by two thermostable beta-glycosidases.
Because a high oligosaccharide concentration prevails until about 80% lactose conversion, thermostable beta-glycosidases are efficient for oligosACcharide production from lactose, and prove to be stable and versatile catalysts for lactose utilization. Expand
Sphingomyelin consumption suppresses aberrant colonic crypt foci and increases the proportion of adenomas versus adenocarcinomas in CF1 mice treated with 1,2-dimethylhydrazine: implications for
Sphingolipids are hydrolyzed in the gastrointestinal tract to ceramide, sphingosine, and other metabolites that can modulate cell growth, differentiation, and apoptosis. To characterize the effectsExpand
The detection of oxycodone in meconium specimens.
A procedure for the determination of oxycodone in meconium using direct ELISA microplate technology followed by electron impact gas chromatography-mass spectrometry (GC-MS) is described for the firstExpand
The importance of charge-separation reactions in tandem mass spectrometry of doubly protonated angiotensin II formed by electrospray ionization: Experimental considerations and structural implications
The results provide evidence with regard to the mechanisms of fragmentation of multiply charged ions and the location of the charge site in relation to the decomposition reactions. Expand
Synthesis and immunochemical characterization of neoglycoproteins containing epitopes of the inner core region of Pseudomonas aeruginosa RNA group I lipopolysaccharide
The disaccharide allyl 2-acetamido-2-deoxy-α-D-galactopyranosyl-(1→3)-7-O-carbamoyl-L- glyceroα-D-manno-heptopyranoside 5 (GalNAc-cmHep-allyl) was synthesized starting from 1 and 2. Compound 5,Expand
Transgalactosylation by thermostable beta-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus. Binding interactions of nucleophiles with the galactosylated enzyme intermediate make
To determine how specificity is manifested under conditions of kinetically controlled transgalactosylation, the major transfer products formed during the hydrolysis of lactose by these enzymes have been identified, and their appearance and degradation have been determined in dependence of the degree of substrate conversion. Expand
Synthesis of inner core antigens related to Chlamydia, Pseudomonas and Acinetobacter LPS
Glycosides of the Kdo-isosteric D-glycero-D-talo-2-octulosonic acid (Ko) occurring in Acinetobacter spp. Expand
Synthesis of neoglycoproteins containing Kdo epitopes specific for Chlamydophila psittaci lipopolysaccharide
The data showed that the ( 2→8)-linked Kdo disaccharide and the (2→8-(2→4)- linked Kdo trisaccharides portion of the neoglycoconjugate 23 were not available for binding of antibodies which recognize these structures as di- and trisACcharide, respectively. Expand
Selective colorimetric detection of Gram-negative re-contaminants in pasteurised milk products by a novel application of the BacT/ALERT 3D system
Based on the technique developed, a faster hygiene monitoring and enhanced approval of dairy product lots at the production site is enabled. Expand