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Selection and characterization of naturally occurring single-domain (IgNAR) antibody fragments from immunized sharks by phage display.
The novel immunoglobulin isotype novel antigen receptor (IgNAR) is found in cartilaginous fish and is composed of a heavy-chain homodimer that does not associate with light chains. The variable… Expand
Host-targeting protein 1 (SpHtp1) from the oomycete Saprolegnia parasitica translocates specifically into fish cells in a tyrosine-O-sulphate–dependent manner
- Stephan Wawra, J. Bain, +10 authors P. van West
- Biology, Medicine
- Proceedings of the National Academy of Sciences
- 20 January 2012
The eukaryotic oomycetes, or water molds, contain several species that are devastating pathogens of plants and animals. During infection, oomycetes translocate effector proteins into host cells,… Expand
The role of host organism, transcriptional switches and reporter mechanisms in the performance of Hg‐induced biosensors
Aims: The purpose of this study was to comprehensively compare the response of nine biosensors capable of being induced by Hg. Induction by Hg was based upon the insertion of merR, merB, zntA and… Expand
Shark variable new antigen receptor biologics – a novel technology platform for therapeutic drug development
- M. Kovaleva, L. Ferguson, J. Steven, A. Porter, C. Barelle
- Business, Medicine
- Expert opinion on biological therapy
- 8 September 2014
Introduction: Biologics drugs have succeeded in achieving a commercial dominance in the global market for new therapies and large pharmaceutical companies' interest remains strong through a continued… Expand
Rapid selection of anti-hapten antibodies isolated from synthetic and semi-synthetic antibody phage display libraries expressed in Escherichia coli.
- G. Strachan, J. McElhiney, M. Drever, F. Mcintosh, L. Lawton, A. Porter
- Biology, Medicine
- FEMS microbiology letters
- 1 May 2002
Antibody phage display libraries (Griffin and Tomlinson I) displaying antibody genes and maintained and amplified in Escherichia coli were used to isolate antibodies to the hapten target microcystin… Expand
Therapeutic Potential of Shark Anti-ICOSL VNAR Domains is Exemplified in a Murine Model of Autoimmune Non-Infectious Uveitis
Induced costimulatory ligand (ICOSL) plays an important role in the activation of T cells through its interaction with the inducible costimulator, ICOS. Suppression of full T cell activation can be… Expand
The isolation of super-sensitive anti-hapten antibodies from combinatorial antibody libraries derived from sheep.
The complexity and expense of producing anti-hapten monoclonals via the traditional hybridoma route and the preferential selection of antibodies that recognise the conjugated form of the hapten, over… Expand
Rapid isolation of IgNAR variable single-domain antibody fragments from a shark synthetic library.
The immunoglobulin isotype IgNAR (Novel Antigen Receptor) was discovered in the serum of the nurse shark (Ginglymostoma cirratum) and wobbegong shark (Orectolobus maculates) as a homodimer of two… Expand
Stabilization of antibody fragments in adverse environments
- H. Dooley, S. D. Grant, W. Harris, A. Porter
- Chemistry, Medicine
- Biotechnology and applied biochemistry
- 1 August 1998
Antibody fragments have the potential to be used as sensitive and specific binding agents in a broad range of industrial applications. Genetic manipulation has been used to design a series of… Expand
Development and application of bioluminescent Caenorhabditis elegans as multicellular eukaryotic biosensors
We describe a novel approach to assess toxicity to the free‐living nematode Caenorhabditis elegans that relies on the ability of firefly luciferase to report on endogenous ATP levels. We have… Expand