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Effect of Cryoconservation on Seed Germination of Rare Tropical Orchids
The feasibility of the conservation of mature seeds of some tropical orchids by freezing in liquid nitrogen was demonstrated, which offers opportunities for creating a cryobank ofOrchids.
Cryopreservation of seeds and protocorms of rare temperate orchids
Germination rates of seeds after their exposure to liquid nitrogen were species-depended and could be either higher or lower than in the unfrozen control, while growth rates of protocorms of the same species obtained from seeds collected in various Russia regions and cultured for 5 months were no significant difference.
The Effect of Seed Cryopreservation on the Development of Protocorms by the Hybrid Orchid Bratonia
Study of the influence of storage in liquid nitrogen on the viability of seeds of the hybrid orchid Bratonia and further development of its protocorms in vitro found that cryopreservation had little effect on the subsequent growth of protOCorms on the agarized MS and in liquid Morel media.
Cryopreservation of Seeds of Some Tropical Orchids
It is shown that cryopreservation (storage in liquid nitrogen at –196 ° C) of seeds, shoot meristems, and cells in vitro could be used in addition to conventional methods of conservation of endangered species of plants.
Cryopreservation of protocorm-like bodies of the hybrid orchid Bratonia (Miltonia flavescens × Brassia longissima).
Investigations into the functional activity of photosystems I and II in PLBs suggest that electron transport was retained in the reaction centers of both photoystems shortly after cryopreservation, and no morphological differences were observed between plants regenerated from Cryopreserved and untreated PLBs.
Photochemical activities of two photosystems in Bratonia orchid protocorms cryopreserved by vitrification method
Functional activities of two photosystems in orchid-specific embryos (protocorms) of a tropical hybrid orchid Bratonia were investigated before and after their cryopreservation by vitrification
Cryopreservation of Digitalis lanata cell cultures.
The results indicate that there is no selection of a special cell type during freezing and thawing and the cultures derived from the surviving cells had the same growth rate and biochemical activity relative to the transformation of cardenolides, e.g., digitoxin, as the parent cultures.
The development of juvenile plants of the hybrid orchid Bratonia after seed cryopreservation.
The development of juvenile plants of hybrid Bratonia orchid in vitro after seed storage in liquid nitrogen and the effect of nutrient medium composition on protocorm multiplication and plant
Cryobank of plant genetic resources in Russian Academy of Sciences
Cryopreservation is the most reliable method for long-term storage of plant genetic resources and 27 cell lines successfully resumed their growth after storage in liquid nitrogen and preserved their specific features and biosynthetic potential.
Recovery of cell cultures and their biosynthetic capacity after storage of Dioscorea deltoidea and Panax ginseng cells in liquid nitrogen
It is shown that the spectrum and amount of steroid compounds, diosgenine in particular, synthesized in Dioscorea suspension cultures do not change after cell storage in liquid nitrogen.