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Characterization of domains in the yeast MAP kinase Slt2 (Mpk1) required for functional activity and in vivo interaction with protein kinases Mkk1 and Mkk2
- M. Soler, A. Plovins, H. Martin, M. Molina, C. Nombela
- Medicine, Biology
- Molecular microbiology
- 1 September 1995
MKK1/MKK2 and SLT2 (MPK1) are three Saccharomyces cerevisiae genes, coding for protein kinases, that have been postulated to act sequentially as part of the Pkc1p signalling pathway, a… Expand
Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells.
- A. Plovins, A. Álvarez, M. Ibáñez, M. Molina, C. Nombela
- Biology, Medicine
- Applied and environmental microbiology
- 1 December 1994
Fluorescein-di-beta-D-galactopyranoside (FDG) was found to be a useful substrate for beta-galactosidase detection by flow cytometry in gram-negative bacteria, since it entered viable cells and gave a… Expand
UseofFluorescein-Di-3 -D-Galactopyranoside (FDG)andC12- FDG asSubstrates for13-Galactosidase Detection byFlow Cytometry inAnimal, Bacterial, andYeastCells
sion of 3-galactosidase incell cultures; butitisnotpossible to monitor expression inindividual cells andtoanalyze the heterogeneity ofexpression incell populations. Theuse ofa fluorogenic substrate,… Expand