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Characterization of domains in the yeast MAP kinase Slt2 (Mpk1) required for functional activity and in vivo interaction with protein kinases Mkk1 and Mkk2
MKK1/MKK2 and SLT2 (MPK1) are three Saccharomyces cerevisiae genes, coding for protein kinases, that have been postulated to act sequentially as part of the Pkc1p signalling pathway, aExpand
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Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells.
Fluorescein-di-beta-D-galactopyranoside (FDG) was found to be a useful substrate for beta-galactosidase detection by flow cytometry in gram-negative bacteria, since it entered viable cells and gave aExpand
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UseofFluorescein-Di-3 -D-Galactopyranoside (FDG)andC12- FDG asSubstrates for13-Galactosidase Detection byFlow Cytometry inAnimal, Bacterial, andYeastCells
sion of 3-galactosidase incell cultures; butitisnotpossible to monitor expression inindividual cells andtoanalyze the heterogeneity ofexpression incell populations. Theuse ofa fluorogenic substrate,Expand