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A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many differentExpand
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The HaeIV restriction modification system of Haemophilus aegyptius is encoded by a single polypeptide.
The HaeIV restriction endonuclease (ENase) belongs to a distinct class of ENases, characterized by its ability to cleave double-stranded DNA on both sides of its recognition sequence, excising aExpand
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Recombination of constant and variable modules alters DNA sequence recognition by type IC restriction‐modification enzymes.
EcoR124 and EcoDXXI are allelic type I restriction‐modification (R‐M) systems whose specificity genes consist of common structural elements: two variable regions are separated by a constant,Expand
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Macroevolution by transposition: drastic modification of DNA recognition by a type I restriction enzyme following Tn5 transposition.
We have characterized a novel mutant of EcoDXXI, a type IC DNA restriction and modification (R‐M) system, in which the specificity has been altered due to a Tn5 insertion into the middle of hsdS, theExpand
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Purification and properties of a new restriction endonuclease from Haemophilus influenzae Rf.
Haemophilus influenzae Rf 232, showing the phenomena of restriction and modification, contains an endonuclease that inactivates in vitro the biological activity of DNAs lacking the strain-specificExpand
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Restriction and modification systems of Neisseria gonorrhoeae.
An individual strain of Neisseria gonorrhoeae may produce up to 16 different DNA methytransferases (MTases). We have used a novel cloning system that is able to detect MTase clones in the absence ofExpand
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The role of Dam methylation in phase variation of Haemophilus influenzae genes involved in defence against phage infection.
Haemophilus influenzae uses phase variation (PV) to modulate the activity of its defence systems against phage infection. The PV of the restriction-modification (R-M) system HindI, the main defenceExpand
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  • Open Access
Biochemical analysis of Lpt3, a protein responsible for phosphoethanolamine addition to lipooligosaccharide of pathogenic Neisseria.
The inner core of neisserial lipooligosaccharide (LOS) contains heptose residues that can be decorated by phosphoethanolamine (PEA). PEA modification of heptose II (HepII) can occur at the 3, 6, or 7Expand
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Characterization of the dsDNA prophage sequences in the genome of Neisseria gonorrhoeae and visualization of productive bacteriophage
Bioinformatic analysis of the genome sequence of Neisseria gonorrhoeae revealed the presence of nine probable prophage islands. The distribution, conservation and function of many of these sequences,Expand
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The DNA sequence recognised by the HinfIII restriction endonuclease.
HinfIII is a type III restriction enzyme (Kauc & Piekarowicz, 1978) isolated from Haemophilus influenzae Rf. Like other type III restriction endonucleases, the enzyme also catalyses the modificationExpand
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