Share This Author
The nuclear receptor homologue Ftz-F1 and the homeodomain protein Ftz are mutually dependent cofactors
It is shown that the orphan nuclear receptor α Ftz-Fl (ref. 3), which is deposited in the egg during oogenesis4, is an obligatory cofactor for Ftz, and this interaction suggests that other nuclear receptor/homeodomain protein interactions maybe important and common in developing organisms.
The interaction with DNA of wild‐type and mutant fushi tarazu homeodomains.
- A. Percival-Smith, M. Müller, M. Affolter, W. Gehring
- Biology, ChemistryThe EMBO journal
- 1 December 1990
The in vitro DNA binding properties of wild‐type and mutant fushi tarazu homeodomains (ftz HD) have been analysed and it is found that the ftz HD and the Antp HD peptides contact DNA in a similar way.
The structure of the homeodomain and its functional implications.
Characterization and mutational analysis of a cluster of three genes expressed preferentially during sporulation of Saccharomyces cerevisiae
The SPS1-SPS2 intergenic region was shown to contain all of the regulatory sequences necessary for the sporulation-specific activation of the SPS2 gene as assessed by expression of a translational SPS 2-lacZ fusion gene present on a replicating, centromere-containing plasmid.
Genetic characterization of the role of the two HOX proteins, Proboscipedia and Sex Combs Reduced, in determination of adult antennal, tarsal, maxillary palp and proboscis identities in Drosophila…
It is proposed that SCR activity is required in the mesodermal adepithelial cells of all leg imaginal discs at late second/early third instar larval stage for the synthesis of a mesoderm-specific, tarsus-inducing, signaling factor, which after secretion from the adep ithelial cells acts on the overlaying ectodermal cells determining tarsu identity.
FTZ‐Factor1 and Fushi tarazu interact via conserved nuclear receptor and coactivator motifs
It is shown that Drosophila FTZ‐F1 does have an AF2 motif and that it is required to bind a coactivator, the homeodomain‐containing protein Fushi tarazu (FTZ), and that FTZ contains an AF 2‐interacting nuclear receptor box, the first to be found in a homeodOMain protein.
Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development
A multicolor imaging tool named Raeppli allows whole-tissue labeling such that the descendants of the majority of cells in a single organ are labeled and can be followed simultaneously relative to one another.
Isolation of DNA sequences preferentially expressed during sporulation in Saccharomyces cerevisiae
A differential hybridization screen has been used to identify genes cloned from the yeast Saccharomyces cerevisiae that are expressed preferentially during sporulation and three genes have been identified that are expression in all cell types during vegetative growth and only in a alpha cells in sporulation medium.
Homeodomain-independent activity of the fushi tarazu polypeptide in Drosophila embryos
It is shown that the 'anti-ftz' phenotype is induced by ectopic expression of a ftz polypeptide containing a deletion in the homeodomain, which can alter gene expression without binding directly to DNA.
DNA binding properties of the purified Antennapedia homeodomain.
- M. Affolter, A. Percival-Smith, M. Müller, W. Leupin, W. Gehring
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 1 June 1990
The results of methylation and ethylation interference studies indicated that the Antp HD closely approaches the target DNA primarily from one side in a region extending across three phosphate backbones.