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Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli Infections
TLDR
Increased application of advanced molecular technologies in clinical laboratories has significantly improved the capacity to diagnose STEC infection early in the course of disease and to detect low levels of environmental contamination, creating a potential window of opportunity for future therapeutic intervention. Expand
Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157.
TLDR
Two multiplex PCR assays for the detection and genetic characterization of STEC in cultures of feces or foodstuffs detected STEC of the appropriate genotype in primary fecal cultures from five patients with hemolytic-uremic syndrome and three with bloody diarrhea. Expand
Molecular Characterization of a Shiga ToxigenicEscherichia coli O113:H21 Strain Lacking eaeResponsible for a Cluster of Cases of Hemolytic-Uremic Syndrome
TLDR
A STEC O113:H21 strain lackingeae was responsible for a cluster of three cases of hemolytic-uremic syndrome and produces a single Stx2-related toxin and adheres efficiently to Henle 407 cells. Expand
Characterization of Saa, a Novel Autoagglutinating Adhesin Produced by Locus of Enterocyte Effacement-Negative Shiga-ToxigenicEscherichia coli Strains That Are Virulent for Humans
TLDR
A gene from the megaplasmid of a LEE-negative O113:H21 STEC strain (98NK2) responsible for an outbreak of HUS encodes an auto-agglutinating adhesin designated Saa, which exhibits a low degree of similarity with YadA of Yersinia enterocolitica and Eib, a recently described phage-encoded immunoglobulin binding protein from E. coli. Expand
Detection and Characterization of Shiga ToxigenicEscherichia coli by Using Multiplex PCR Assays forstx1, stx2,eaeA, Enterohemorrhagic E. coli hlyA,rfbO111, andrfbO157
TLDR
Two multiplex PCR assays for the detection and genetic characterization of STEC in cultures of feces or foodstuffs detected STEC of the appropriate genotype in primary fecal cultures from five patients with hemolytic-uremic syndrome and three with bloody diarrhea. Expand
A New Family of Potent AB5 Cytotoxins Produced by Shiga Toxigenic Escherichia coli
TLDR
Findings suggest that the toxin secreted by the Shiga toxigenic Escherichia coli O113:H21 strain 98NK2 may contribute to the pathogenesis of human disease. Expand
Opposing unfolded-protein-response signals converge on death receptor 5 to control apoptosis
TLDR
It is reported that unmitigated ER stress promoted apoptosis through cell-autonomous, UPR-controlled activation of death receptor 5 (DR5), which integrates opposing UPR signals to couple ER stress and apoptotic cell fate. Expand
Direct Detection and Characterization of Shiga Toxigenic Escherichia coli by Multiplex PCR for stx1, stx2, eae, ehxA, and saa
  • A. Paton, J. Paton
  • Medicine, Biology
  • Journal of Clinical Microbiology
  • 1 January 2002
TLDR
This study developed a pentavalent PCR assay for the detection of saa as well as other proven and putative STEC virulence genes (stx1, stx2, eae, and ehxA), which do not interfere with each other and generate amplification products of 119, 180, 255, 384, and 534 bp. Expand
Molecular microbiological investigation of an outbreak of hemolytic-uremic syndrome caused by dry fermented sausage contaminated with Shiga-like toxin-producing Escherichia coli
TLDR
During an outbreak of HUS in Adelaide, South Australia, a sensitive PCR assay specific for Shiga-like toxin genes (slt) was used to test cultures of feces and suspected foods, indicating that at least three different O111:H- genotypes were associated with the outbreak. Expand
AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP
TLDR
BiP is a master regulator of endoplasmic reticulum function, and its cleavage by subtilase cytotoxin represents a previously unknown trigger for cell death. Expand
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