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Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
TLDR
A method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction using a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer.
G1 events and regulation of cell proliferation.
  • A. Pardee
  • Biology, Chemistry
    Science
  • 3 November 1989
TLDR
This work has shown that switches in and out of G1 are the main determinants of post-embryonic cell proliferation rate and are defectively controlled in cancer cells.
A restriction point for control of normal animal cell proliferation.
  • A. Pardee
  • Biology
    Proceedings of the National Academy of Sciences…
  • 1 April 1974
TLDR
Evidence is given here that cells are put into the same quiescent state by each of these diverse blocks to proliferation and that cells escape at the same point in G(1) of the cell cycle when nutrition is restored.
Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization.
TLDR
It is shown that the number of anchored oligo-dT primers can be reduced from twelve to four that are degenerate at the penultimate base from the 3' end, which enables further streamlining of the technique and make it readily applicable to a broad spectrum of biological systems.
The enzymology of control by feedback inhibition.
The Restriction Point of the Cell Cycle
TLDR
The relationship between the restriction knot and DNA damage-checkpoints is discussed, and how loss of the restriction point in cancer leads to loss of checkpoint control and to insensitivity to antimitogens including some mechanism-based anticancer therapeutics.
Suppression of cancer relapse and metastasis by inhibiting cancer stemness
TLDR
It is shown that BBI608, a small molecule identified by its ability to inhibit gene transcription driven by Stat3 and cancer stemness properties, can inhibit stemness gene expression and block spherogenesis of or kill stemness-high cancer cells isolated from a variety of cancers.
NF-kappa B activation in human breast cancer specimens and its role in cell proliferation and apoptosis.
TLDR
The hypothesis that certain breast cancer cells rely on NF-kappa B for aberrant cell proliferation and simultaneously avoid apoptosis is substantiated, thus implicating activated NF- kappa B as a therapeutic target for distinctive subclasses of ER-negative breast cancers.
Synchronization of tumor and normal cells from G1 to multiple cell cycles by lovastatin.
TLDR
Lovastatin appears to prevent formation of an early intermediate in the cholesterol pathway that is essential for progression of cells through early G1 phase of the cell cycle.
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