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Abiotic Stress Generates ROS That Signal Expression of Anionic Glutamate Dehydrogenases to Form Glutamate for Proline Synthesis in Tobacco and Grapevine[W]
Glutamate dehydrogenase (GDH) may be a stress-responsive enzyme, as GDH exhibits considerable thermal stability, and de novo synthesis of the α-GDH subunit is induced by exogenous ammonium andExpand
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Reduced activity of antioxidant machinery is correlated with suppression of totipotency in plant protoplasts.
We previously showed that during protoplast isolation, an oxidative burst occurred and the generation of active oxygen species was differentially mediated in tobacco (Nicotiana tabacum) and grapevineExpand
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Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide
Our previous results indicate that during protoplast isolation an oxidative burst occurs [A.K. Papadakis and KA Roubelakis-Angelakis (1999) Plant Physiol 127:197–205] and that suppression ofExpand
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The generation of active oxygen species differs in tobacco and grapevine mesophyll protoplasts.
Our previous results have shown that oxidative stress may reduce the regeneration potential of protoplasts, but only protoplasts that are able to supply extracellularly H(2)O(2) can actually divideExpand
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Oxidative stress could be responsible for the recalcitrance of plant protoplasts
Expression of totipotency and regeneration potentiality of plant protoplasts is a complex developmental phenomenon. The isolation per se is a stress-inducing procedure, during which, among others,Expand
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Biosynthesis profile and endogenous titers of polyamines differ in totipotent and recalcitrant plant protoplasts
The expression of totipotency in plant protoplasts is a complex developmental phenomenon and is affected by genetic and physiological factors. Polyamines (PAs) are known to be involved in a varietyExpand
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Virological fitness of HIV in patients with resistance to enfuvirtide
Resistance to the HIV fusion inhibitor enfuvirtide is associated with mutations in the first heptad repeat region of gp41, but little is known of their impact on replicative fitness in vivo. WeExpand
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Prevalence of transmitted HIV drug resistance since the availability of highly active antiretroviral therapy.
We investigated the prevalence of HIV-1-associated transmitted drug resistance (TDR) in Victoria from the time of first availability of highly active antiretroviral therapy. Drug resistanceExpand
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Protoplast Technology in Grapevine
Hastein (1880) was probably the first to use the term protoplast, from the greek proto (πρώτο = first) and plastos (πλαστός = created), for plant cells lacking cell walls and to isolate protoplastsExpand
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