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Mycolactone subverts immunity by selectively blocking the Sec61 translocon
TLDR
This identifies the most potent Sec61 inhibitor reported to date and the potential of inhibiting Sec61 for immune modulation.
Mutational Analysis of the Role of Nucleoside Triphosphatase P4 in the Assembly of the RNA Polymerase Complex of Bacteriophage φ6
ABSTRACT Bacteriophage φ6 is a complex enveloped double-stranded RNA virus with a segmented genome and replication strategy quite similar to that of the Reoviridae. An in vitro packaging and
Proteomics Reveals Scope of Mycolactone-mediated Sec61 Blockade and Distinctive Stress Signature*
TLDR
Quantitative proteomics reveals that Sec61 blockade induces proteostatic stress in the cytosol and the endoplasmic reticulum, refining the mechanistic understanding of Sec61 inhibition by mycolactone.
A symmetry mismatch at the site of RNA packaging in the polymerase complex of dsRNA bacteriophage phi6.
TLDR
This is the first example of dsRNA virus in which the structure of the polymerase complex has been determined without the assumption of icosahedral symmetry, and illustrates the symmetry mismatch which may occur at the sites of RNA packaging in other ds RNA viruses such as members of the Reoviridae.
Nonspecific Nucleoside Triphosphatase P4 of Double-Stranded RNA Bacteriophage φ6 Is Required for Single-Stranded RNA Packaging and Transcription
TLDR
Surprisingly, a mutation in P4, S250Q, which reduced the level of P4 in the particles to about 10% of the wild-type level, did not affect RNA packaging activity or change the kinetics of packaging.
Double-stranded RNA bacteriophage phi 6 protein P4 is an unspecific nucleoside triphosphatase activated by calcium ions
TLDR
The nucleotide specificity and enzymatic properties of the P4 NTPase are similar to the NTP hydrolysis reaction conditions needed to translocate and condense the viral positive-sense RNAs to the procapsid particle.
Bacteriophage Mu integration in yeast and mammalian genomes
TLDR
New DNA is introduced into eukaryotic cells using bacteriophage Mu DNA transposition complexes, termed ‘transpososomes’, to clarify the constraints exerted by genome structure on genomic parasites, and illustrate the wide utility of the Mu transpososome technology for gene transfer in eukARYotic cells.
Crystal Structure of an Engineered LRRTM2 Synaptic Adhesion Molecule and a Model for Neurexin Binding.
TLDR
The structural and functional data presented here provide the first structure of an LRRTM protein and allow us to propose a model for the molecular mechanism ofLRRTM function in the synaptic adhesion.
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