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A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria
TLDR
A new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli is developed, which is especially useful for site–directed transposon mutagenesis and for site-specific gene transfer in a wide variety of gramnegative organisms.
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum.
TLDR
Small mobilizable vectors based on the Escherichia coli plasmids pK18 and pK19 can be transferred by RP4-mediated conjugation into a wide range of Gram- and Gram+ bacteria, and should facilitate gene disruption and allelic exchange by homologous recombination.
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.
TLDR
These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil.
The Composite Genome of the Legume Symbiont Sinorhizobium meliloti
TLDR
The annotated DNA sequence of the α-proteobacteriumSinorhizobium meliloti, the symbiont of alfalfa, is presented, indicating that all three elements contribute, in varying degrees, to symbiosis and reveals how this genome may have emerged during evolution.
GenDB--an open source genome annotation system for prokaryote genomes.
TLDR
A genome annotation system for prokaryote genomes, which is well tested and readily adaptable to different tasks, and can be employed as a flexible framework for the large-scale evaluation of different annotation strategies.
Genomics of IncP-1 antibiotic resistance plasmids isolated from wastewater treatment plants provides evidence for a widely accessible drug resistance gene pool.
TLDR
Findings derived from genomic analysis of IncP-1 resistance plasmids isolated from WWTP bacteria reveal that animal, human and plant pathogens and other bacteria isolated from different habitats share a common pool of resistance determinants.
Overlaps in the Transcriptional Profiles of Medicago truncatula Roots Inoculated with Two Different Glomus Fungi Provide Insights into the Genetic Program Activated during Arbuscular Mycorrhiza1[w]
TLDR
This study focused on the overlapping genetic program activated by two commonly studied microsymbionts in addition to identifying AM-related genes, and demonstrated that one member of the AM-induced gene family encoding blue copper binding proteins was both specifically and strongly up-regulated in arbuscule-containing regions of mycorrhizal roots.
EDGAR: A software framework for the comparative analysis of prokaryotic genomes
TLDR
EDGAR provides novel analysis features and significantly simplifies the comparative analysis of related genomes and supports a quick survey of evolutionary relationships and simplifying the process of obtaining new biological insights into the differential gene content of kindred genomes.
Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis
TLDR
The genome sequence provides the basis for the future design of strategies to mitigate the ecological damage caused by oil spills and provides A. borkumensis SK2 with a competitive edge in oil-polluted environments.
TRANSGENIC ROOT-NODULES OF VICIA-HIRSUTA - A FAST AND EFFICIENT SYSTEM FOR THE STUDY OF GENE-EXPRESSION IN INDETERMINATE-TYPE NODULES
TLDR
The Agrobacterium rhizogenes-mediated transformation of Vicia hirsuta resulted in the induction of transgenic 'hairy roots' on wounded epicotyls of young seedlings, and composite plants were established that consisted of transgen roots on untransformed shoots.
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