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First detailed quantification of silymarin components in the leaves of Silybum marianum cultivated in egypt during different growth stages
Summary A high performance liquid chromatographic method was developed for the quantitative determination of the active components of silymarin in the leaves of Silybum marianum during different…
Somatic Embryogenesis: Still a Relevant Technique in Citrus Improvement.
This chapter provides a brief history of plant somatic embryogenesis with focus on citrus, followed by a discussion of proven applications in biotechnology-facilitated citrus improvement techniques, such as somatic hybridization, somatic cybridization, genetic transformation, and the exploitation of somaclonal variation.
Salinity Tolerance of ‘Valencia’ Orange Trees on Allotetraploid Rootstocks
Three allotetraploid zygotic hybrid rootstock selections were able to exclude Na+ and Cl− ions from ‘Valencia’ leaves better than most other rootstocks while maintaining good growth with no phytotoxic symptoms and merit further evaluation of salinity tolerance and horticultural performance in the field.
Enhanced resistance to citrus canker in transgenic mandarin expressing Xa21 from rice
- A. Omar, M. Murata, H. El-Shamy, J. Graham, J. Grosser
- Biology, MedicineTransgenic Research
- 14 February 2018
Genetic engineering approaches offer an alternative method to the conventional breeding of Citrus sp. ‘W. Murcott’ mandarin (a hybrid of ‘Murcott’ and an unknown pollen parent) is one of the most…
Estimation of transgene copy number in transformed citrus plants by quantitative multiplex real‐time PCR
Quantitative real‐time PCR (qRT‐PCR) was adapted to estimate transgene copy number of the rice Xa21 gene in transgenic citrus plants and can be performed at much earlier stages of transgenic plant development than southern blot analysis, which expedites investigation of transgenes in slow‐growing woody plants.
Introduction of Xa21, a Xanthomonas-resistance gene from rice, into ‘Hamlin’ sweet orange [Citrus sinensis (L.) Osbeck] using protoplast-GFP co-transformation or single plasmid transformation
This is the first report of a large population of transgenic ‘Hamlin’ sweet orange plants containing one or more target gene(s), using a protoplast-GFP transformation system, to avoid the use of Agrobacterium, antibiotic resistance genes, and destructive assay systems.
Metabolically speaking: Possible reasons behind the tolerance of 'Sugar Belle' mandarin hybrid to huanglongbing.
Efficacy Comparison of Some New Natural-Product Herbicides for Weed Control at Two Growth Stages
Abstract There is an urgent need to accelerate the development and implementation of effective organic-compliant herbicides that are environmentally safe and that help the producer meet increasing…
Melatonin Regulatory Mechanisms and Phylogenetic Analyses of Melatonin Biosynthesis Related Genes Extracted from Peanut under Salinity Stress
The results indicated that melatonin regulated the redox homeostasis by its ability to induce either enzymatic or non-enzymatic antioxidant systems.
Progress Towards the Development of Transgenic Disease Resistance in Citrus
This work has successfully cloned several natural and synthetic antibacterial genes and genes that have the potential to turn on SAR (Systemic Acquired Resistance) and made significant progress in introducing them into commercial sweet orange and grapefruit cultivars.