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Cloning and sequencing of a high-alkaline pectate lyase gene from an alkaliphilic Bacillus isolate.
Pel-103 appeared to have a similar core and active site topology to the enzymes of known structure from Erwinia chrysanthemi and Bacillus subtilis, and physicochemical and catalytic properties of Pel-103 were different from those of other enzymes reported so far.
Nucleotide and amino-acid sequences of a new-type pectate lyase from an alkaliphilic strain of Bacillus.
Based on their amino-acid sequence homology, Pel-15E and PelA appear to belong to a new class of Pel family, although the enzymatic properties of both enzymes were quite different.
Endoglucanases from Paenibacillus spp. form a new clan in glycoside hydrolase family 5.
Endoglucanase (Egl)-producing bacteria from soil samples were screened using insoluble cellulosic substrates as sole carbon sources at alkaline pH (pH 9-10) and recombinant enzyme degraded lichenan, carboxymethylcellulose (CMC), glucomannan, acid or alkaline swollen celluloses, and microcrystalline cellulose (Avicel).
A new subtilisin family: nucleotide and deduced amino acid sequences of new high-molecular-mass alkaline proteases from Bacillus spp.
Six genes encoding high-molecular-mass subtilisins (HMSs) of alkaliphilic Bacillus spp. were cloned and sequenced. Their open reading frames of 2,394–2,424 bp encoded prosubtilisins of 798–808 amino
Production of neutral protease from Aspergillus oryzae by a novel cultivation method on a microporous membrane
A novel cultivation method using a microporous membrane (membrane-surface liquid culture) was developed, in which moulds are grown on the membrane surface with its opposite side being in contact with
A New High-alkaline and High-molecular-weight Pectate Lyase from a Bacillus Isolate: Enzymatic Properties and Cloning of the Gene for the Enzyme
A pectate lyase found in an alkaline culture of Bacillus sp.
Production of kojic acid from Aspergillus oryzae var. oryzae by membrane-surface liquid culture
Membrane-surface liquid culture (MSLC) developed by the authors (Yasuhara et al., 1994) was applied to the production of kojic acid, using Aspergillus oryzae var.oryzae IFO 30113. By the fed-batch