MITOCHONDRIAL LOCALIZATION OF OXIDATIVE ENZYMES: STAINING RESULTS WITH TWO TETRAZOLIUM SALTS
It is concluded that, except for formazan deposition on lipid-aqueous interfaces, Nitro-BT staining indicates the intracellular localization of oxidative enzymes, at least at the level of light microscopy, and the use of MTT-Co++ is not reliable for such intrACEllular localizations.
VISUALIZATION OF PEROXISOMES (MICROBODIES) AND MITOCHONDRIA WITH DIAMINOBENZIDINE
Manganese iotis (0.001 .1!) stimulate peroxisome staining, i)ut manganese precipitates from solution at p11 9.0 or below, and their stimulatory effect can be demonstrated readily.
Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytological studies.
An examination of scale models can readily demonstrate that no other bonding scheme permits both the glycosidic bonds of the G-C pair to be simultaneously superimposed on those of the AT pair of structure XII.
Organelle relationships in cultured 3T3-L1 preadipocytes
The morphological differentiation of 3T3-L1 preadipocytes includes the loss of "stress fibers" and the appearance of microfilament like structures that encase, in a complex manner, the cytosolic lipid spheres that appear during differentiation.
Lysosomes and Related Particles
- A. Novikoff
- Biology, Chemistry
ELECTRON MICROSCOPY OF THE HUMAN SYNOVIAL MEMBRANE
The structure of the lining cells at the surface of the synovial membrane facing the joint cavity has been studied by electron microscopy and the probable functions of these cells are discussed in the light of current knowledge of the metabolism and function of the Synovial membranes.
LYSOSOMES IN THE RAT SCIATIC NERVE FOLLOWING CRUSH
Peripheral nerves undergoing degeneration are favorable material for studying the types, origins, and functions of lysosomes, and the breakdown of organelles within these vacuoles may have significance for the reorganization of the axoplasm preparatory to regeneration.
PEROXISOMES IN ABSORPTIVE CELLS OF MAMMALIAN SMALL INTESTINE
The term "microperoxisomes" is proposed to distinguish these peroxisomal proteins of rat hepatocytes from the better-known larger peroxISomes of liver and kidney, which resemble the small perox isomes described in many other cell types.
THE LOCALIZATION OF PHOSPHATASE ACTIVITIES AT THE LEVEL OF ULTRASTRUCTURE
- S. Goldfischer, E. Essner, A. Novikoff
- BiologyJournal of Histochemistry and Cytochemistry
- 1 February 1964
Significant intracellular localizations can now be described with coulsideral (-onfidenc(-, as imidicated in Text Fig. 1).
GOLGI APPARATUS, GERL, AND LYSOSOMES OF NEURONS IN RAT DORSAL ROOT GANGLIA, STUDIED BY THICK SECTION AND THIN SECTION CYTOCHEMISTRY
Serial 0.5-µ and thin sections of thiamine pyrophosphatase-incubated material demonstrate that, in the neurons studied, the Golgi apparatus is a continuous network coursing through the cytoplasm, and suggest that GERL is also a continuous structure throughout the cy toplasm.