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Membrane fusion plays an important role in gene transfection mediated by cationic liposomes
The results suggest that at least two steps are effective for gene transfection mediated by the cationic liposomes with cATIONic cholesterol derivatives, including the endocytosis of the liposome/DNA complex into the target cells and the removal of antisense oligonucleotides (plasmid DNAs) from the complex in the endosomes. Expand
Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative
From the results it was found that the vesicles with moderate diameters were moste effective for gene transfection of plasmid pSV2CAT DNA into the target cell. Expand
Confocal and probe microscopy to study gene transfection mediated by cationic liposomes with a cationic cholesterol derivative.
A novel cationic cholesterol derivative with a hydroxyethyl amino head group (I) has been synthesized and used for liposome-mediated gene transfection and it was found that microtubules were involved in the intracellular dynamics of genetransfection. Expand
Cationic Cholesterol Promotes Gene Transfection Using the Nuclear Localization Signal in Protamine
Atomic force microscopy and fluorescence microscopic images showed that Cy5-labeled antisense DNAs were transferred quicker into the nucleus of the target cells by the liposomes with the derivative I in the presence of protamine. Expand
Activation of receptor tyrosine kinases promotes gene transfection in rat neuronal PC12 cells by cationic liposomes
It is supposed that the novel liposomes with the derivative (I) will be useful to study the problems in neuron and neuronal tissues for gene transfection and depended on the activation of receptor tyrosine kinases. Expand
[Gene transfection mediated by cationic liposomes with a cationic cholesterol derivative].