• Publications
  • Influence
Function, mechanism and regulation of bacterial ribonucleases.
  • A. Nicholson
  • Medicine, Biology
  • FEMS microbiology reviews
  • 1 June 1999
The maturation and degradation of RNA molecules are essential features of the mechanism of gene expression, and provide the two main points for post-transcriptional regulation. Cells employ aExpand
  • 244
  • 13
Ribonuclease III mechanisms of double-stranded RNA cleavage
  • A. Nicholson
  • Medicine, Biology
  • Wiley interdisciplinary reviews. RNA
  • 30 September 2013
Double‐stranded(ds) RNA has diverse roles in gene expression and regulation, host defense, and genome surveillance in bacterial and eukaryotic cells. A central aspect of dsRNA function is itsExpand
  • 107
  • 8
Structure, reactivity, and biology of double-stranded RNA.
  • A. Nicholson
  • Biology, Medicine
  • Progress in nucleic acid research and molecular…
  • 1996
Publisher Summary This chapter focuses on the structure and physicochemical properties of double stranded RNA (dsRNA); on the enzymes that degrade, modify, or otherwise modulate dsRNA structure andExpand
  • 102
  • 6
Molecular characterization of a mouse cDNA encoding Dicer, a ribonuclease III ortholog involved in RNA interference
Abstract. Members of the ribonuclease III superfamily of double-stranded(ds)-RNA-specific endoribonucleases participate in diverse cellular RNA maturation and degradation pathways. A recentlyExpand
  • 75
  • 6
Regulation of ribonuclease III processing by double-helical sequence antideterminants.
  • K. Zhang, A. Nicholson
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 9 December 1997
The double helix is a ubiquitous feature of RNA molecules and provides a target for nucleases involved in RNA maturation and decay. Escherichia coli ribonuclease III participates in maturation andExpand
  • 96
  • 5
  • PDF
Bacteriophage T7 protein kinase phosphorylates RNase E and stabilizes mRNAs synthesized by T7 RNA polymerase
The T7 protein encoded by the early gene 0.7 exhibits bifunctional activity. Whereas its C‐terminal one‐third participates in host transcription shut‐off, the N‐terminal two‐thirds bears a proteinExpand
  • 55
  • 5
Mechanism of action of Escherichia coli ribonuclease III. Stringent chemical requirement for the glutamic acid 117 side chain and Mn2+ rescue of the Glu117Asp mutant.
Escherichia coli ribonuclease III (EC 3.1.24) is a double-strand- (ds-) specific endoribonuclease involved in the maturation and decay of cellular, phage, and plasmid RNAs. RNase III is a homodimerExpand
  • 47
  • 5
Mutational analysis of the nuclease domain of Escherichia coli ribonuclease III. Identification of conserved acidic residues that are important for catalytic function in vitro.
The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. BacterialExpand
  • 33
  • 5
Ribonuclease III cleavage of a bacteriophage T7 processing signal. Divalent cation specificity, and specific anion effects.
Escherichia coli ribonuclease III, purified to homogeneity from an overexpressing bacterial strain, exhibits a high catalytic efficiency and thermostable processing activity in vitro. The RNaseExpand
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Defining the enzyme binding domain of a ribonuclease III processing signal. Ethylation interference and hydroxyl radical footprinting using catalytically inactive RNase III mutants.
Ethylation interference and hydroxyl radical footprinting were used to identify substrate ribose‐phosphate backbone sites that interact with the Escherichia coli RNA processing enzyme, ribonucleaseExpand
  • 67
  • 4