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Superresolution Fluorescence Imaging of Mitochondrial Nucleoids Reveals Their Spatial Range, Limits, and Membrane Interaction
ABSTRACT A fundamental objective in molecular biology is to understand how DNA is organized in concert with various proteins, RNA, and biological membranes. Mitochondria maintain and express theirExpand
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Replication of mitochondrial DNA occurs by strand displacement with alternative light-strand origins, not via a strand-coupled mechanism.
The established strand-displacement model for mammalian mitochondrial DNA (mtDNA) replication has recently been questioned in light of new data using two-dimensional (2D) agarose gel electrophoresis.Expand
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Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR
Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensingExpand
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Movement and structure of mitochondria in oligodendrocytes and their myelin sheaths
Mitochondria play several crucial roles in the life of oligodendrocytes. During development of the myelin sheath they are essential providers of carbon skeletons and energy for lipid synthesis.Expand
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Facile and General Synthesis of Photoactivatable Xanthene Dyes**
Despite the apparent simplicity of the xanthene fluorophores, the preparation of caged derivatives with free carboxy groups remains a synthetic challenge. A straightforward and flexible strategy forExpand
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Native R-loops Persist throughout the Mouse Mitochondrial DNA Genome*S⃞
Mammalian mtDNA has been found here to harbor RNA-DNA hybrids at a variety of locations throughout the genome. The R-loop, previously characterized in vitro at the leading strand replication originExpand
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Approaches toward super-resolution fluorescence imaging of mitochondrial proteins using PALM.
Mitochondria are difficult targets for microscopy because of their small size and highly compartmentalized, membranous interior. Super-resolution fluorescence microscopy methods have recently beenExpand
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Rational Design of Fluorogenic and Spontaneously Blinking Labels for Super-Resolution Imaging
Rhodamine dyes exist in equilibrium between a fluorescent zwitterion and a nonfluorescent lactone. Tuning this equilibrium toward the nonfluorescent lactone form can improve cell-permeability andExpand
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mechanism with alternative light-strand origins , not via a strand-coupled Replication of mitochondrial DNA occurs by strand displacement data
data Supplementary http://www.genesdev.org/cgi/content/full/19/20/2466/DC1 "Supplemental Research Data" References http://www.genesdev.org/cgi/content/full/19/20/2466#References This article cites 41Expand
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General Synthetic Method for Si-Fluoresceins and Si-Rhodamines
The century-old fluoresceins and rhodamines persist as flexible scaffolds for fluorescent and fluorogenic compounds. Extensive exploration of these xanthene dyes has yielded generalExpand
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