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Cloning of a gluconate/polyol dehydrogenase gene from Gluconobacter suboxydans IFO 12528, characterisation of the enzyme and its use for the production of 5-ketogluconate in a recombinant Escherichia
A 5-ketogluconate (5-KGA)-forming membrane quinoprotein, gluconate dehydrogenase, was isolated from Gluconobacter suboxydans strain IFO 12528 and partially sequenced. Partial sequences of fiveExpand
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Biochemical and genetic characterization of a novel enzyme of pentitol metabolism: D-arabitol-phosphate dehydrogenase.
An enzyme with a specificity that has not been described previously, D-arabitol-phosphate dehydrogenase (APDH), has been purified from cell lysate of Enterococcus avium. SDS/PAGE indicated that theExpand
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Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d-Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols
ABSTRACT Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar d-ribose from d-glucose in a single fermentation step are described. AExpand
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Production of xylitol by metabolically engineered strains of Bacillus subtilis.
Xylitol-phosphate dehydrogenase (XPDH) genes from several Gram-positive bacteria were isolated and expressed in Bacillus subtilis. The substrate specificities of the recombinant XPDH enzymes wereExpand
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Cloning and Characterization of Gluconolactone Oxidase of Penicillium cyaneo-fulvum ATCC 10431 and Evaluation of Its Use for Production of d-Erythorbic Acid in Recombinant Pichia pastoris
ABSTRACT A d-erythorbic acid-forming soluble flavoprotein, gluconolactone oxidase (GLO), was purified from Penicillium cyaneo-fulvum strain ATCC 10431 and partially sequenced. Peptide sequences wereExpand
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Characterization of a novel endo-levanase and its gene from Bacillus sp. L7.
A novel endo-levanase producing bacterium belonging to the Bacillus family has been isolated from soil. The enzyme was characterized and found to have no exo-beta-fructofuranosidase activity. TheExpand
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Synthesis of arabinitol 1-phosphate and its use for characterization of arabinitol-phosphate dehydrogenase.
D-arabinitol 1-phosphate (Ara-ol1-P), a substrate for D-arabinitol-phosphate dehydrogenase (APDH), was chemically synthesized from D-arabinonic acid in five steps (O-acetylation, chlorination,Expand
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[Human resistance to decompression sickness and nonspecific methods of its elevation].
The object of this study was the dependence of decompression sickness (DCS) tolerance determined by the intensity of venous gas embolism on functioning of the cardiovascular and respiratory systems,Expand
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Characterisation of a secondary alcohol dehydrogenase from Xanthomonas campestris DSM 3586
The chromosomal locus NP_636946 of Xanthomonas campestris DSM 3586 (ATCC 33913) which was earlier presumed to encode a quinoprotein glucose dehydrogenase has been cloned, expressed in EscherichiaExpand
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[Preparation of Saccharomyces cerevisiae strains with regulated MFalpha1 promotor activity].
Two Saccharomyces cerevisiae strains containing integrated copies of the MAT alpha 1 gene fused to the PHO5 promoter have been obtained by transformation of a MAT alpha 1 mutant. The strains differExpand