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Molecular tagging of erucic acid trait in oilseed mustard (Brassica juncea) by QTL mapping and single nucleotide polymorphisms in FAE1 gene
TLDR
Molecular mapping and tagging of the erucic acid trait (C22:1) in Brassica juncea was done by a candidate gene approach and four substitution type single nucleotide polymorphisms (SNPs) in FAE1.1 and three in FAe1.2 were identified.
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A high-density linkage map in Brassica juncea (Indian mustard) using AFLP and RFLP markers
TLDR
The map developed in the present study could be used for dissection and the transfer of agronomically important traits and favourable QTLs from ill-adapted exotic germplasm to cultivated Indian varieties.
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Heterosis breeding in Indian mustard (Brassica juncea L. Czern & Coss): Analysis of component characters contributing to heterosis for yield
TLDR
Estimation of heterosis from BYP was a more accurate method to determine the contribution of component characters towards yield heterosis than the analysis based on MP and BP.
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Variation amongst Brassica juncea cultivars for regeneration from hypocotyl explants and optimization of conditions for Agrobacterium-mediated genetic transformation
TLDR
Plants resistant to 20 μg/ml hygromycin were regenerated at a frequency of 11–36% from hypocotyl explants and were shown to be transformed by Southern blotting, GUS staining and progeny analysis.
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Mapping of yield influencing QTL in Brassica juncea: implications for breeding of a major oilseed crop of dryland areas
TLDR
Quantitative trait loci analysis of yield influencing traits was carried out in Brassica juncea using a doubled haploid mapping population derived from a cross between Varuna and Heera to identify potentially useful alleles from both the parents, suggesting a more prominent role of the A genome towards domestication of this crop.
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Natural mutations in two homoeologous TT8 genes control yellow seed coat trait in allotetraploid Brassica juncea (AABB)
TLDR
It is hypothesized that the mutations in both the TT8 genes are required for inhibiting the transcription of LBGs in the yellow-seeded mutant of B. juncea.
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Development of high oleic and low linoleic acid transgenics in a zero erucic acid Brassica juncea L. (Indian mustard) line by antisense suppression of the fad2 gene
TLDR
Analysis of the levels of linoleic acid (C18:2) in the BC1 seeds of single-copy transgenics showed that the construct pASfad2.2 gave better suppression of the fad2 gene as compared to the construct PMF2.1.
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A passage through in vitro culture leads to efficient production of marker-free transgenic plants in Brassica juncea using the Cre–loxP system
TLDR
It is shown that a passage through in vitro culture of F1 leaf explants allows efficient development of marker-free transgenics in the F2 generation addressing current limitations associated with efficient use of the Cre/loxP technology for marker gene removal.
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Molecular mapping reveals two independent loci conferring resistance to Albugocandida in the east European germplasm of oilseed mustard Brassica juncea
TLDR
Two independent loci governing resistance to A. candida race 2V are tagged in two east European lines, Heera and Donskaja-IV, to assist introgression of resistance-conferring loci in the susceptible varieties of B. juncea.
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Targeted silencing of BjMYB28 transcription factor gene directs development of low glucosinolate lines in oilseed Brassica juncea.
TLDR
The development of transgenic B. juncea lines having seed glucosinolates as low as 11.26 μmol/g DW, through RNAi-based targeted suppression of BjMYB28, a R2R3-MYB transcription factor family gene involved in aliphatic glucos inolate biosynthesis is reported.
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