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Cloning, sequencing, purification, and crystal structure of Grenache (Vitis vinifera) polyphenol oxidase.
The full-length cDNA sequence (P93622_VITVI) of polyphenol oxidase (PPO) cDNA from grape Vitis vinifera L., cv Grenache, was found to encode a translated protein of 607 amino acids with an expected
Crystal Structure of Ovocleidin-17, a Major Protein of the Calcified Gallus gallus Eggshell
Determining the three-dimensional structure of OC17 contributes to a better understanding of the biological behavior of structurally related biomolecules and of the mechanisms involved in eggshell and other mineralization processes.
High resolution X‐ray crystallographic structure of bovine heart cytochrome c and its application to the design of an electron transfer biosensor
The purification, crystallization and 3D structure of one of the cytochrome c isoforms is presented to 1.5 Å resolution and it is shown how the presence of isoforms made both the purification and crystallization steps difficult.
Enzymatic polymerization of phenolic compounds using laccase and tyrosinase from Ustilago maydis.
The results showed that the laccase- and tyrosinase-catalyzed polymerization of quercetin produced aggregates with relatively low molecular weight and higher antioxidant activity than the monomeric quercETin.
Crystal growth of proteins, nucleic acids, and viruses in gels.
On Electrochemically Assisted Protein Crystallization and Related Methods
In this communication, the basic strategies to control the kinetics and transport phenomena of the crystal growth process, as well as novel methods to induce either nucleation or protein crystal
Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth
In this contribution we use nonconventional methods that help to increase the success rate of a protein crystal growth, and consequently of structural projects using X-ray diffraction techniques. In