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Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
It is shown that the first approach can lead to PCR efficiencies that vary over a 0.2 range, whereas the second approach may be off by 0.26, and proposed linear regression on the Log(fluorescence) per cycle number data as an assumption-free method to calculate starting concentrations of mRNAs and PCRefficiencies for each sample. Expand
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA transcripts, most analyses of qPCR data are still delegated to the software that comes with the qPCR apparatus. ThisExpand
The transcriptional repressor Tbx3 delineates the developing central conduction system of the heart.
The analysis implicates a role for Tbx3 in repressing a chamber-specific program of gene expression in regions from which the components of the central conduction system are subsequently formed. Expand
Cooperative action of Tbx2 and Nkx2.5 inhibits ANF expression in the atrioventricular canal: implications for cardiac chamber formation.
The T-box family member Tbx2, encoding a transcriptional repressor, is expressed in the embryonic myocardium in a pattern mutually exclusive to ANF, thus suggesting a role in the suppression of ANF. Expand
Chamber formation and morphogenesis in the developing mammalian heart.
The spatial pattern of expression of genes encoding atrial natriuretic factor, sarcoplasmic reticulum calcium ATPase, Chisel,Irx5, Irx4, myosin light chain 2v, and beta-myosin heavy chain are traced and a two-step model for chamber formation in the embryonic heart is proposed. Expand
T‐box transcription factor Tbx2 represses differentiation and formation of the cardiac chambers
Findings provide the first evidence that Tbx2 is a determinant in the local repression of chamber‐specific gene expression and chamber differentiation. Expand
Cardiac chamber formation: development, genes, and evolution.
The topographical arrangement of the distinct cardiac muscle cells in the forming heart explains the embryonic electrocardiogram (ECG), does not require the invention of nodes, and allows a logical transition from a peristaltic tubular heart to a synchronously contracting four-chambered heart. Expand
WHSC1, a 90 kb SET domain-containing gene, expressed in early development and homologous to a Drosophila dysmorphy gene maps in the Wolf-Hirschhorn syndrome critical region and is fused to IgH in
Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4 (4p16.3), and WHSC1 is proposed as a good candidate gene to be responsible for many of the phenotypic features of WHS. Expand
Tbx3 controls the sinoatrial node gene program and imposes pacemaker function on the atria.
It is shown that Tbx3 expression delineates the sinoatrial node region, which runs a gene expression program that is distinct from that of the bordering atrial cells as soon as cardiac cells turn on the atrial gene Expression program. Expand