• Publications
  • Influence
Macromolecular crowding and confinement: biochemical, biophysical, and potential physiological consequences.
TLDR
Theoretical and experimental approaches to the characterization of crowding- and confinement-induced effects in systems approaching the complexity of living organisms are suggested.
The Influence of Macromolecular Crowding and Macromolecular Confinement on Biochemical Reactions in Physiological Media*
  • A. Minton
  • Chemistry, Medicine
    The Journal of Biological Chemistry
  • 6 April 2001
TLDR
To properly assess the physiological role of a particular reaction or set of reactions characterized in vitro, it is important to consider the possible influence of crowding and/or confinement upon the reaction in its physiological milieu.
Macromolecular crowding: biochemical, biophysical, and physiological consequences.
TLDR
This paper presents a meta-analyses of the macromolecular determinants of reaction rates and volume in a solution and describes the mechanisms leading to these rates and volumes.
Excluded volume as a determinant of macromolecular structure and reactivity
The effect of excluded volume on the thermodynamic activity of globular macromolecules and macromolecular complexes in solution is studied in the hard‐particle approximation. Activity coefficients
Models for excluded volume interaction between an unfolded protein and rigid macromolecular cosolutes: macromolecular crowding and protein stability revisited.
  • A. Minton
  • Chemistry, Medicine
    Biophysical journal
  • 1 February 2005
Statistical-thermodynamic models for the excluded volume interaction between an unfolded polypeptide chain and a hard sphere or hard rod cosolute are presented, permitting estimation of the free
Effect of dextran on protein stability and conformation attributed to macromolecular crowding.
TLDR
Addition of high concentrations of dextran to solutions of acid-unfolded cytochrome c at pH 2.0 results in a shift of the CD spectrum from that characteristic of the fully unfolded polypeptide to that characteristics of the more compact, salt-induced molten globule state, a result suggesting that the molten globules-like state is stabilized relative to the fully unfolding form in crowded environments.
Cell biology: Join the crowd
Cells are packed with large molecules. The ramifications of this 'crowding' for a wide range of intracellular processes are only now becoming more generally understood.
Macromolecular crowding: qualitative and semiquantitative successes, quantitative challenges.
The concept of excluded volume and the theory of effects of excluded volume on the equilibria and rates of macromolecular reactions in fluid media containing high total concentrations of
The effect of volume occupancy upon the thermodynamic activity of proteins: some biochemical consequences
  • A. Minton
  • Chemistry, Medicine
    Molecular and Cellular Biochemistry
  • 2004
TLDR
Experimental studies of the effect of ‘inert’ macromolecules on protein structure and/or function are reviewed, and it is found that under favorable circumstances the simplified models can provide a satisfactory semiquantitative description of the data.
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