• Publications
  • Influence
The Lipid Structure of the Glycosylphosphatidylinositol-anchored Mucin-like Sialic Acid Acceptors of Trypanosoma cruzi Changes during Parasite Differentiation from Epimastigotes to Infective
Results indicate that when epimastigotes transform into infective metacyclic trypomastigote Trypanosoma cruzi forms, the phosphatidylinositol moiety of the glycosylphosphatidol inositol anchor of the major acceptor of sialic acid is modified, while the glycoproteins and O-linked sugar chains remain essentially unchanged. Expand
Overexpression of the plasma membrane-localized NDR1 protein results in enhanced bacterial disease resistance in Arabidopsis thaliana.
Sequence analysis and mass spectrometry suggest that NDR1 is localized to the PM via a C-terminal glycosylphosphatidyl-inositol (GPI) anchor, perhaps allowing NDR 1 to act as a transducer of pathogen signals and/or interact directly with the pathogen. Expand
Lipoarabinomannan from Mycobacterium tuberculosis induces the production of tumour necrosis factor from human and murine macrophages.
We show here that purified lipoarabinomannan (LAM) from Mycobacterium tuberculosis can cause the release of tumour necrosis factor (TNF) in vitro from human blood monocytes and activated mouseExpand
The Phosphoproteome of Bloodstream Form Trypanosoma brucei, Causative Agent of African Sleeping Sickness
Analysis of the cytosolic bloodstream form of T. brucei revealed the presence of 44 phosphorylated protein kinases in the data set that could be classified into the major eukaryotic protein kinase groups by applying a multilevel hidden Markov model library of the kinase catalytic domain. Expand
The inhibitory effects of mycobacterial lipoarabinomannan and polysaccharides upon polyclonal and monoclonal human T cell proliferation.
Lipoarabinomannan from Mycobacterium tuberculosis was able to inhibit antigen induced T cell proliferation of human CD4+ T cell clones specific for influenza virus. The inhibitory effect was alsoExpand
The glycoforms of a Trypanosoma brucei variant surface glycoprotein and molecular modeling of a glycosylated surface coat.
A molecular model of the glycoprotein is constructed to assess the role of N-linked oligosaccharides in the architecture of the surface coat of the African sleeping sickness parasite Trypanosoma brucei and it is shown that VSG MITat.1.5 is glycosylated at all three potential N-glycosylation sites, and the oligosACcharides present at each site are assigned. Expand
Deletion of the Glucosidase II Gene in Trypanosoma brucei Reveals Novel N-Glycosylation Mechanisms in the Biosynthesis of Variant Surface Glycoprotein*
The creation of a bloodstream-form T. brucei mutant that is deficient in the endoplasmic reticulum enzyme glucosidase II is described and points to fundamental differences in the specificities of host and parasite glycosyltransferases that initiate the synthesis of complex N-glycans. Expand
Modeling of the N-Glycosylated Transferrin Receptor Suggests How Transferrin Binding Can Occur within the Surface Coat of Trypanosoma brucei
This model, when placed in the context of a model for the dense variant surface glycoprotein coat in which it is embedded, suggests that receptor N-glycosylation may play an important role in providing sufficient space for the approach and binding of transferrin to the receptor, without significantly disrupting the continuity of the protective variant surface carbohydrate coat. Expand
The quantitative variability and monosaccharide composition of sediment carbohydrates associated with intertidal diatom assemblages
The extracellular secretions of epipelic diatoms (Bacillariophyceae) axe an important source of carbohydrates on intertidal sediments. For analytical purposes, sediment carbohydrates have beenExpand
Biochemical and antigenic characterization of the Mycobacterium tuberculosis 71 kD antigen, a member of the 70 kD heat‐shock protein family
Biochemical and serological characterization of the protein confirms its membership of the hsp70 gene family, and metabolic labelling demonstrates that it is a major component of the mycobacterial response to heat stress. Expand