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Sequencing end-labeled DNA with base-specific chemical cleavages.
TLDR
The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions. Expand
A new method for sequencing DNA.
  • A. Maxam, W. Gilbert
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 February 1977
TLDR
Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described. Expand
Mapping adenines, guanines, and pyrimidines in RNA.
TLDR
The positions of adenines, guanines, and pyrimidines can be determined by partial nuclease digestion of a terminally labeles RNA molecule and form the basis of an RNA sequencing method and are demonstrated on yeast 5.8S ribosomal RNA. Expand
Cloning, sequencing, and functional analysis of oriL, a herpes simplex virus type 1 origin of DNA synthesis.
The herpes simplex virus type 1 genome (160 kilobases) contains three origins of DNA synthesis: two copies of oriS located within the repeated sequences flanking the short unique arm (US), and oneExpand
A T cell‐specific enhancer is located in a DNase I‐hypersensitive area at the 3′ end of the CD3‐delta gene.
TLDR
Findings suggest that regulatory elements 3′ of the CD3‐delta gene are at least in part responsible for its exclusive expression in thymus‐derived lymphocytes. Expand
The nucleotide sequence of the lac operator.
  • W. Gilbert, A. Maxam
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences…
  • 1 December 1973
TLDR
The sequence of RNA transcription copies of this fragment is determined and the sequence has 2-fold symmetry regions; the two longest are separated by one turn of the DNA double helix. Expand
Rous sarcoma virus genome is terminally redundant: the 5' sequence.
TLDR
The existence of this sequence repetition immediately suggests mechanisms by which the growing DNA copy can jump from the 5' end to a 3' end of the template and become circular. Expand
The T cell receptor V alpha 3 gene segment is associated with reactivity to p-azobenzenearsonate.
TLDR
It is suggested that V alpha 3 encodes a protein sequence with a binding site for the arsonate hapten that confers reactivity to arsonate in association with at least three different MHC proteins (I-Ad, I-Ak, and Ld). Expand
Sequence of a mouse germ-line gene for a variable region of an immunoglobulin light chain.
TLDR
The sequence confirms that the variable region gene lies on the DNA separated from the constant region of a mouse immunoglobulin light chain, the VlambdaII gene, andHypervariable region codons appear in the germ-line sequence. Expand
Methylation of protein by calf spleen methylase. A new protein methylation reaction.
TLDR
Chemical studies have revealed that spleen protein methylase, unlike other known protein methylases, methylates an amino acid residue other than lysine or arginine, and, therefore, it represents a new enzymatic protein methylation reaction. Expand
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