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Pyridoxal 5'phosphate binding site of Escherichia coli beta cystathionase and cystathionine gamma synthase comparison of their sequences.
The phosphopyridoxyl peptides of beta cystathionase and cystathionine gamma synthase of Escherichia Coli were identified after reduction, carboxymethylation and proteolysis of the holoenzymes. TheirExpand
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Mass Spectrometric Determination of the Cleavage Sites inEscherichia coli Dihydroorotase Induced by a Cysteine-specific Reagent*
Escherichia coli dihydroorotase contains six cysteines/subunit, which are potential ligands of structural and catalytic zinc metals at protein sites of the enzyme. Specific thiol reagents modify, inExpand
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Kinetic studies of aminoglycoside acetyltransferase and phosphotransferase from Staphylococcus aureus RPAL. Relationship between the two activities.
In the Staphylococcus aureus strain harbouring the plasmid RPAL, the resistance to aminoglycoside antibiotics results from two inactivating reactions catalyzed by a 6'-N-aminoglycosideExpand
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(Eta6-arene) ruthenium(II) complexes and metallo-papain hybrid as Lewis acid catalysts of Diels-Alder reaction in water.
Covalent embedding of a (eta(6)-arene) ruthenium(II) complex into the protein papain gives rise to a metalloenzyme displaying a catalytic efficiency for a Lewis acid-mediated catalysed Diels-AlderExpand
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Occurrence of mycotoxin patulin in apple-based products marketed in Tunisia.
An investigation on the presence of mycotoxin patulin was performed on 71 apple juices and concentrates and 21 infant fruit purees purchased from retail outlets or producers in Tunisia, byExpand
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Assay of Escherichia coli dihydroorotase with enantiomeric substrate: practical preparation of carbamyl L-aspartate and high-performance liquid chromatography analysis of catalysis product.
A reasonably facile and effective procedure is described for preparing the optically active substrate of dihydroorotase (EC, N-carbamyl-L-aspartate (L-CA), which is not commerciallyExpand
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Generation and characterization of polyclonal antibodies against microcystins-Application to immunoassays and immunoaffinity sample preparation prior to analysis by liquid chromatography and UV
Polyclonal antibodies against microcystin-LR (MC-LR), a cyclic heptapeptide toxin, were generated in rabbits using MC-LR-BSA. An enzyme-linked immunosorbent assay (ELISA) was developed for theExpand
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Purification and characterization of the main pepsinogen from the shark, Centroscymnus coelolepis.
The main pepsinogen from the mucosa of the shark, Centroscymnus coelolepis, has been purified and characterized. This zymogen, the most abundant protein in terms of quantity and activity (yield 72%),Expand
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Cysteine‐Specific, Covalent Anchoring of Transition Organometallic Complexes to the Protein Papain from Carica papaya
Site‐directed and covalent introduction of various transition metal–organic entities to the active site of the cysteine endoproteinase, papain, was achieved by treatment of this enzyme with a seriesExpand
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Role of subunit interactions in the self-assembly of oligomeric proteins.
In oligomeric proteins, the native conformation and its functional properties depend on the interactions which exist between the different chains. The role of these subunit interactions can beExpand
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