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All ras proteins are polyisoprenylated but only some are palmitoylated
The C-terminal CAAX motif of the yeast mating factors is modified by proteolysis to remove the three terminal amino acids (-AAX) leaving a C-terminal cysteine residue that is polyisoprenylated andExpand
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Aggregation of Lipid Rafts Accompanies Signaling via the T Cell Antigen Receptor
The role of lipid rafts in T cell antigen receptor (TCR) signaling was investigated using fluorescence microscopy. Lipid rafts labeled with cholera toxin B subunit (CT-B) and cross-linked intoExpand
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The cadherin superfamily: diversity in form and function.
Over recent years cadherins have emerged as a growing superfamily of molecules, and a complex picture of their structure and their biological functions is becoming apparent. Variation in theirExpand
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The role of lipid rafts in T cell antigen receptor (TCR) signalling.
Plasma membranes of many cell types contain domains enriched in specific lipids and cholesterol, called lipid rafts. In T lymphocytes, key T cell antigen receptor (TCR) signalling molecules associateExpand
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A Salmonella typhimurium Effector Protein SifA Is Modified by Host Cell Prenylation and S-Acylation Machinery*
SifA is a Salmonella effector protein that is required for maintenance of the vacuolar membrane that surrounds replicating bacteria. It associates with the Salmonella-containing vacuole but how itExpand
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Rab GTPases Containing a CAAX Motif Are Processed Post-geranylgeranylation by Proteolysis and Methylation*
Post-translational modification by protein prenylation is required for membrane targeting and biological function of monomeric GTPases. Ras and Rho proteins possess a C-terminal CAAX motif (C isExpand
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Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells.
We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered andExpand
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S‐acylation of LCK protein tyrosine kinase is essential for its signalling function in T lymphocytes
LCK is a non‐receptor protein tyrosine kinase required for signal transduction via the T‐cell antigen receptor (TCR). LCK N‐terminus is S‐acylated on Cys3 and Cys5, in addition to its myristoylationExpand
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Protein acyl thioesterases (Review)
Many proteins are S-acylated, affecting their localization and function. Dynamic S-acylation in response to various stimuli has been seen for several proteins in vivo. The regulation of S-acylationExpand
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Post‐translational processing of p21ras is two‐step and involves carboxyl‐methylation and carboxy‐terminal proteolysis.
We have studied the post‐translational processing of p21ras proteins. The primary translation product pro‐p21 is cytosolic and is rapidly converted to a cytosolic form (c‐p21) of higher mobility onExpand
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