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Cleavage of cellulose by a CBM33 protein
It is shown that some members of the CBM33 family cleave crystalline cellulose as demonstrated by chromatographic and mass spectrometric analyses of soluble products released from Avicel or filter paper on incubation with CelS2, a CBM 33‐containing protein from Streptomyces coelicolor A3(2). Expand
Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases
The structural and functional characterization of two functionally coupled cellulose-active LPMOs belonging to auxiliary activity family 10 (AA10) that commonly occur in cellulolytic bacteria are described, concluding that substrate specificity depends not on copper site architecture, but rather on variation in substrate binding and orientation. Expand
Metagenomics of the Svalbard Reindeer Rumen Microbiome Reveals Abundance of Polysaccharide Utilization Loci
Lignocellulosic biomass remains a largely untapped source of renewable energy predominantly due to its recalcitrance and an incomplete understanding of how this is overcome in nature. We present hereExpand
Clavulanic acid dehydrogenase: structural and biochemical analysis of the final step in the biosynthesis of the beta-lactamase inhibitor clavulanic acid.
Biophysical analyses demonstrate that CAD exists as dimeric and tetrameric species in solution and suggests how CAD is able to catalyze the reduction of clavulanate-9-aldehyde without fragmentation of the bicyclic beta-lactam ring structure. Expand
A Polysaccharide Utilization Locus from an Uncultured Bacteroidetes Phylotype Suggests Ecological Adaptation and Substrate Versatility
The presence of a PUL that possesses saccharolytic activity against a mixture of abundantly available polysaccharides supports the dominance of SRM-1 in the Svalbard reindeer rumen microbiome. Expand
Discovery and characterization of a thermostable two-domain GH6 endoglucanase from a compost metagenome
Functional data and the crystal structure, solved at 1.88 Å resolution, indicate that mgCel6A is an endoglucanase, and comparative studies showed that the CBM increases substrate binding, while not affecting thermal stability, underpinning previous suggestions that CBMs may be less useful in high-consistency bioprocessing. Expand
Crystal structures exploring the origins of the broader specificity of escherichia coli heat-labile enterotoxin compared to cholera toxin.
Important molecular determinants underlying the different binding specificities of LTB and CTB are found to be contributed by Ser95, Tyr18 and Thr4 (or Ser4 of hLTB), which together prestabilize the binding site by positioning Lys91, Glu51 and the adjacent loop region containing Ile58 for ligand binding. Expand
Comprehensive analysis of blood group antigen binding to classical and El Tor cholera toxin B-pentamers by NMR.
Detailed analysis of ABH blood group determinants using saturation transfer difference NMR provides a framework for the interpretation of the epidemiological data linking the severity of cholera infection and an individual's blood group, and brings us one step closer to understanding the molecular basis of Cholera blood group dependence. Expand
The activities of nucleoside diphosphate kinase and adenylate kinase are influenced by their interaction
Evidence of an interaction between the plant mitochondrial NDPK and a plant mitochondrial adenylate kinase is presented, which could be an unchanged ADP production, albeit with different rates of substrate consumption. Expand
Two SusD-Like Proteins Encoded within a Polysaccharide Utilization Locus of an Uncultured Ruminant Bacteroidetes Phylotype Bind Strongly to Cellulose
Two characteristic Sus-like proteins encoded within a polysaccharide utilization locus bind strongly to cellulosic substrates and interact with plant primary cell walls, showing associations between uncultured Bacteroidetes-affiliated lineages and cellulose in the rumen. Expand