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Structural and functional conservation of key domains in InsP3 and ryanodine receptors
Inositol-1,4,5-trisphosphate receptors (InsP3Rs) and ryanodine receptors (RyRs) are tetrameric intracellular Ca2+ channels. In each of these receptor families, the pore, which is formed byExpand
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Analysis of protein-ligand interactions by fluorescence polarization
Quantification of the associations between biomolecules is required both to predict and understand the interactions that underpin all biological activity. Fluorescence polarization (FP) provides aExpand
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CaBP1, a neuronal Ca2+ sensor protein, inhibits inositol trisphosphate receptors by clamping intersubunit interactions
Calcium-binding protein 1 (CaBP1) is a neuron-specific member of the calmodulin superfamily that regulates several Ca2+ channels, including inositol 1,4,5-trisphosphate receptors (InsP3Rs). CaBP1Expand
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Subtype-selective regulation of IP3 receptors by thimerosal via cysteine residues within the IP3-binding core and suppressor domain
IP3R (IP3 [inositol 1,4,5-trisphosphate] receptors) and ryanodine receptors are the most widely expressed intracellular Ca2+ channels and both are regulated by thiol reagents. In DT40 cells stablyExpand
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Structural organization of signalling to and from IP3 receptors.
In the 30 years since IP3 (inositol 1,4,5-trisphosphate) was first shown to release Ca2+ from intracellular stores, the importance of spatially organized interactions within IP3-regulated signallingExpand
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Synthetic partial agonists reveal key steps in IP3 receptor activation
Inositol 1,4,5-trisphosphate receptors (IP3R) are ubiquitous intracellular Ca2+ channels. IP3binding to the IP3-binding core (IBC) near the N-terminal initiates conformational changes that lead toExpand
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Binding of Inositol 1,4,5-trisphosphate (IP3) and Adenophostin A to the N-Terminal region of the IP3 Receptor: Thermodynamic Analysis Using Fluorescence Polarization with a Novel IP3 Receptor Ligand
Inositol 1,4,5-trisphosphate (IP3) receptors (IP3R) are intracellular Ca2+ channels. Their opening is initiated by binding of IP3 to the IP3-binding core (IBC; residues 224–604 of IP3R1) andExpand
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Adenophostins: High-Affinity Agonists of IP(3) Receptors.
Publisher Summary Adenophostins are naturally occurring high-affinity agonists of inositol 1,4,5-trisphosphate receptors (IP3R). The three components of adenophostin—bisphosphorylated glucose,Expand
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Selective determinants of inositol 1,4,5-trisphosphate and adenophostin A interactions with type 1 inositol 1,4,5-trisphosphate receptors
BACKGROUND AND PURPOSE Adenophostin A (AdA) is a potent agonist of inositol 1,4,5‐trisphosphate receptors (IP3R). AdA shares with IP3 the essential features of all IP3R agonists, namely structuresExpand
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Activation of IP3 receptors requires an endogenous 1-8-14 calmodulin-binding motif
Binding of IP3 (inositol 1,4,5-trisphosphate) to the IP3-binding core (residues 224–604) of IP3Rs (IP3 receptors) initiates opening of these ubiquitous intracellular Ca2+ channels. The mechanisms areExpand
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