• Publications
  • Influence
Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO2‐based 3‐hydroxypropionate production
TLDR
The results here support the prospects of using thermally diverse sources of pathways and enzymes in metabolically engineered strains designed for product formation at sub‐optimal growth temperatures.
Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea.
TLDR
A reaction kinetics model was developed to examine the biological and biotechnological attributes of the 3HP/4HB cycle as it operates in Metallosphaera sedula and assess metabolic engineering strategies for incorporating CO2 into chemical intermediates and products of bioteschnological importance: acetyl-CoA, succinate, and 3-hydroxypropionate.
Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3‐hydroxypropionate by metabolically engineered Pyrococcus furiosus
TLDR
The results illustrate the ancillary benefits of accessory enzymes for incorporating CO2 into 3HP production in metabolically engineered P. furiosus, and hint at the important role that CA and BPL likely play in the native 3HP/4HB pathway in M. sedula.
Production of lignofuels and electrofuels by extremely thermophilic microbes
TLDR
Recent progress towards the synthesis of lignofuels and electrofuels by extremely thermophilic microorganisms is reviewed, including temperature-regulated biosynthesis of industrial organic chemicals and liquid fuel molecules.
Angular Measurement Error Due to Different Measuring Devices
TLDR
Any one orthopaedic surgeon should use the same goniometer at all times, preferably a fixed type, so that measurement variability can be reduced by ±2.0 degrees.
Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals
TLDR
The issues and opportunities for extremely thermophilic metabolic engineering platforms are considered with an eye toward potential technological advantages for high temperature industrial biotechnology.
A hybrid synthetic pathway for butanol production by a hyperthermophilic microbe.
TLDR
A synthetic metabolic pathway assembled from genes obtained from three different sources for conversion of acetyl-CoA to 1-butanol was demonstrated near 70°C in a microorganism that grows optimally near 100°C, and the module could also be used in thermophiles capable of degrading plant biomass.
Genus-Wide Assessment of Lignocellulose Utilization in the Extremely Thermophilic Genus Caldicellulosiruptor by Genomic, Pangenomic, and Metagenomic Analyses
TLDR
It is indicated that microcrystalline cellulose degradation at temperatures above 70°C, based on current information, is limited to certain members of this genus that produce GH48 domain-containing enzymes.
Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
TLDR
More-selective thermophilic versions of Bad, Bdh, and AdhE are needed to fully exploit biocatalytic n-butanol production at elevated temperatures.
...
1
2
...