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Proteins involved in electron transfer to Fe(III) and Mn(IV) oxides by Geobacter sulfurreducens and Geobacter uraniireducens.
TLDR
The results demonstrate that the physiology of Fe(III)-reducing bacteria differs significantly during growth on different insoluble and soluble electron acceptors and emphasize the importance of c-type cytochromes for extracellular electron transfer in G. sulfurreducens.
Yeast ribonucleotide reductase has a heterodimeric iron-radical-containing subunit.
TLDR
Using highly purified recombinant yeast RNR proteins, it is demonstrated that the crucial role of Rnr4p (beta') is to fold correctly and stabilize the radical-storing Rnr2p by forming a stable 1:1 RNR2p/Rnr 4p complex.
The anaerobic (class III) ribonucleotide reductase from Lactococcus lactis. Catalytic properties and allosteric regulation of the pure enzyme system.
TLDR
The results with the L. lactis class III reductase further support the concept of a common origin for all present day ribonucleotide reductases.
The tyrosyl free radical of recombinant ribonucleotide reductase from Mycobacterium tuberculosis is located in a rigid hydrophobic pocket.
TLDR
Theoretical modeling studies based on the structural data known for Salmonella typhimurium class Ib RNR protein R2F revealed a hydrophobic wall aligned with the radical harboring residue Y110, indicating that the Fe3+ ions also had become reduced by dithionite.
Resolution, Detection, and Characterization of Redox Conformers of Human HSF1*
  • D. Manalo, A. Liu
  • Biology, Chemistry
    The Journal of Biological Chemistry
  • 29 June 2001
TLDR
It is shown that diamide, a reagent that promotes disulfide bond formation, caused a loss of immunorecognition of the monomeric hHSF1 protein in a standard Western blot detection procedure, which suggests that h HSF1 may have integrated redox chemistry of cysteine sulfhydryl into its functional responses.
Human lens thioltransferase: cloning, purification, and function.
TLDR
The human lens TTase gene was cloned for the first time and its gene product showed the characteristics which support speculation that TTase may play a major role in maintaining the homeostasis of lens protein thiols thus protecting against oxidative stress.
The LIS1-related Protein NUDF of Aspergillus nidulans and Its Interaction Partner NUDE Bind Directly to Specific Subunits of Dynein and Dynactin and to α- and γ-Tubulin*
TLDR
Examination of the interactions of the A. nidulans NUDF and NUDE proteins with components of dynein, dynactin, and with α- and γ-tubulin suggests a direct role for NU DF in regulation of the dyne in heavy chain and an effect on other dyneIn/dynactin subunits via N UDE.
Crystal Structure of the cGMP-dependent Protein Kinase II Leucine Zipper and Rab11b Protein Complex Reveals Molecular Details of G-kinase-specific Interactions*♦
TLDR
This is the first structure of PKG bound to one of its interacting proteins, andStructural comparison with PKG Iα and Iβ LZs combined with mutagenic analysis reveals that GKIP recognition is mediated through surface charge interactions.
New paramagnetic species formed at the expense of the transient tyrosyl radical in mutant protein R2 F208Y of Escherichia coli ribonucleotide reductase.
TLDR
The results suggest that Z is most likely an oxo-ferryl species possibly in equilibrium with a Y208 ligand radical, and may be a mainly metal centered species.
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